Volume 1073 2013

Synthetic Biology

Editors:

ISBN: 978-1-62703-624-5 (Print) 978-1-62703-625-2 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-x

  2. Introduction

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-6

      What Is Synthetic Biology?

  3. DNA Assembly

    1. Front Matter

      Pages 7-7

    2. No Access

      Book Protocol

      Pages 9-17

      Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program

    3. No Access

      Book Protocol

      Pages 19-30

      BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit

    4. No Access

      Book Protocol

      Pages 31-42

      Overlap Extension PCR Cloning

    5. No Access

      Book Protocol

      Pages 43-47

      One-Step Isothermal Assembly of DNA Fragments

  4. Part, Pathway, and Genome Engineering

    1. Front Matter

      Pages 49-49

    2. No Access

      Book Protocol

      Pages 51-60

      Creation and Characterization of Component Libraries for Synthetic Biology

    3. No Access

      Book Protocol

      Pages 61-74

      In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements

    4. No Access

      Book Protocol

      Pages 75-84

      In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli

    5. No Access

      Book Protocol

      Pages 85-106

      Construction and Engineering of Large Biochemical Pathways via DNA Assembler

    6. No Access

      Book Protocol

      Pages 107-129

      Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries Through ePathBrick Vectors

    7. No Access

      Book Protocol

      Pages 131-140

      Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research

    8. No Access

      Book Protocol

      Pages 141-156

      Combinatorial DNA Assembly Using Golden Gate Cloning

    9. No Access

      Book Protocol

      Pages 157-168

      Construction of Synthetic Gene Circuits in the Escherichia coli Genome

    10. No Access

      Book Protocol

      Pages 169-174

      Shuffling of DNA Cassettes in a Synthetic Integron

  5. Computational Tools for Modelling Biological Systems

    1. Front Matter

      Pages 175-175

    2. No Access

      Book Protocol

      Pages 177-190

      Systematic Methodology for the Development of Mathematical Models for Biological Processes

    3. No Access

      Book Protocol

      Pages 191-224

      SOBOLHDMR: A General-Purpose Modeling Software

  6. Back Matter

    Pages 225-226