Volume 1063 2013

Membrane Proteins

Folding, Association, and Design

Editors:

ISBN: 978-1-62703-582-8 (Print) 978-1-62703-583-5 (Online)

Table of contents (13 protocols)

  1. Front Matter

    Pages i-x

  2. Association of Transmembrane Helices

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-18

      Use of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments

    3. No Access

      Book Protocol

      Pages 19-36

      Measurement of Transmembrane Peptide Interactions in Liposomes Using Förster Resonance Energy Transfer (FRET)

    4. No Access

      Book Protocol

      Pages 37-56

      Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping

    5. No Access

      Book Protocol

      Pages 57-91

      Genetic Systems for Monitoring Interactions of Transmembrane Domains in Bacterial Membranes

  3. Interactions with the Lipid Bilayer

    1. Front Matter

      Pages 93-93

    2. No Access

      Book Protocol

      Pages 95-116

      Analyzing the Effects of Hydrophobic Mismatch on Transmembrane α-Helices Using Tryptophan Fluorescence Spectroscopy

    3. No Access

      Book Protocol

      Pages 117-124

      Folding Alpha-Helical Membrane Proteins into Liposomes In Vitro and Determination of Secondary Structure

    4. No Access

      Book Protocol

      Pages 125-142

      Solvation Models and Computational Prediction of Orientations of Peptides and Proteins in Membranes

  4. NMR Methods

    1. Front Matter

      Pages 143-143

    2. No Access

      Book Protocol

      Pages 145-158

      Membrane Protein Structure Determination: Back to the Membrane

    3. No Access

      Book Protocol

      Pages 159-180

      On the Role of NMR Spectroscopy for Characterization of Antimicrobial Peptides

  5. Engineering Approaches

    1. Front Matter

      Pages 181-181

    2. No Access

      Book Protocol

      Pages 183-196

      Prediction and Design of Outer Membrane Protein–Protein Interactions

    3. No Access

      Book Protocol

      Pages 197-210

      Design of Transmembrane Peptides: Coping with Sticky Situations

    4. No Access

      Book Protocol

      Pages 211-225

      Engineering and Utilization of Reporter Cell Lines for Cell-Based Assays of Transmembrane Receptors

    5. No Access

      Book Protocol

      Pages 227-243

      Fluorination in the Design of Membrane Protein Assemblies

  6. Back Matter

    Pages 245-246