Volume 1048 2013

Biological Aging

Methods and Protocols

Editors:

ISBN: 978-1-62703-555-2 (Print) 978-1-62703-556-9 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-10

    Cell Senescence Culturing Methods

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    Pages 11-18

    Digital Image Analysis of Cells Stained with the Senescence-Associated β-Galactosidase Assay

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    Pages 19-29

    Analysis of Biomarkers of Caloric Restriction in Aging Cells

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    Pages 31-47

    Cell Sorting of Young and Senescent Cells

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    Pages 49-63

    Studying the Replicative Life Span of Yeast Cells

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    Pages 65-75

    Methods for Creating Mutations in C. elegans That Extend Lifespan

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    Pages 77-93

    Aging Studies in Drosophila Melanogaster

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    Pages 95-107

    The Use of Calorie Restriction Mimetics to Study Aging

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    Pages 109-126

    Using Somatic-Cell Nuclear Transfer to Study Aging

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    Pages 127-133

    Induction of Cellular Senescence by Oncogenic RAS

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    Pages 135-144

    Methods of Cellular Senescence Induction Using Oxidative Stress

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    Pages 145-160

    Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice

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    Pages 161-180

    Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors

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    Pages 181-193

    Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration

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    Pages 195-213

    Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging

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    Pages 215-228

    Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequencing

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    Pages 229-246

    Gel Electrophoresis-Based Proteomics of Senescent Tissues

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    Pages 247-284

    Single-Cell Semiconductor Sequencing

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    Pages 285-308

    Application of DNA Microarray Technology to Gerontological Studies

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    Pages 309-321

    Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells

  22. Back Matter

    Pages 353-354

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