Volume 1020 2013

Guanylate Cyclase and Cyclic GMP

Methods and Protocols

Editors:

ISBN: 978-1-62703-458-6 (Print) 978-1-62703-459-3 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-16

    NO/cGMP: The Past, the Present, and the Future

  3. No Access

    Book Protocol

    Pages 17-50

    cGMP-Dependent Protein Kinases (cGK)

  4. No Access

    Book Protocol

    Pages 51-62

    Enzyme Assays for cGMP Hydrolyzing Phosphodiesterases

  5. No Access

    Book Protocol

    Pages 63-72

    Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues

  6. No Access

    Book Protocol

    Pages 73-88

    Hyperspectral Imaging of FRET-Based cGMP Probes

  7. No Access

    Book Protocol

    Pages 89-120

    Visualization of cGMP with cGi Biosensors

  8. No Access

    Book Protocol

    Pages 121-129

    Advances and Techniques to Measure cGMP in Intact Cardiomyocytes

  9. No Access

    Book Protocol

    Pages 131-145

    Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells

  10. No Access

    Book Protocol

    Pages 147-162

    Methods for Identification of cGKI Substrates

  11. No Access

    Book Protocol

    Pages 163-173

    Approaches for Monitoring PKG1α Oxidative Activation

  12. No Access

    Book Protocol

    Pages 175-192

    Analysis of cGMP Signaling in Adipocytes

  13. No Access

    Book Protocol

    Pages 193-204

    A Genetic Strategy for the Analysis of Individual Axon Morphologies in cGMP Signalling Mutant Mice

  14. No Access

    Book Protocol

    Pages 205-214

    Receptor Binding Assay for NO-Independent Activators of Soluble Guanylate Cyclase

  15. No Access

    Book Protocol

    Pages 215-221

    Direct Intrathecal Drug Delivery in Mice for Detecting In Vivo Effects of cGMP on Pain Processing

  16. No Access

    Book Protocol

    Pages 223-233

    The Geisler Method: Tracing Activity-Dependent cGMP Plasticity Changes upon Double Detection of mRNA and Protein on Brain Slices

  17. No Access

    Book Protocol

    Pages 235-245

    Detection of cGMP in the Degenerating Retina

  18. Back Matter

    Pages 247-249