Volume 1017 2013

Tandem Repeats in Genes, Proteins, and Disease

Methods and Protocols

Editors:

ISBN: 978-1-62703-437-1 (Print) 978-1-62703-438-8 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-20

    Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins

  3. No Access

    Book Protocol

    Pages 21-40

    Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces

  4. No Access

    Book Protocol

    Pages 41-57

    Morphometric Analysis of Huntington’s Disease Neurodegeneration in Drosophila

  5. No Access

    Book Protocol

    Pages 59-71

    Size Analysis of Polyglutamine Protein Aggregates Using Fluorescence Detection in an Analytical Ultracentrifuge

  6. No Access

    Book Protocol

    Pages 73-83

    A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins

  7. No Access

    Book Protocol

    Pages 85-93

    Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation

  8. No Access

    Book Protocol

    Pages 95-104

    Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders

  9. No Access

    Book Protocol

    Pages 105-120

    PCR Amplification and Sequence Analysis of GC-Rich Sequences: Aristaless-Related Homeobox Example

  10. No Access

    Book Protocol

    Pages 121-133

    Challenges of “Sticky” Co-immunoprecipitation: Polyalanine Tract Protein–Protein Interactions

  11. No Access

    Book Protocol

    Pages 135-151

    Molecular Pathology of Polyalanine Expansion Disorders: New Perspectives from Mouse Models

  12. No Access

    Book Protocol

    Pages 153-161

    Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation

  13. No Access

    Book Protocol

    Pages 163-171

    Immuno-based Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological Samples

  14. No Access

    Book Protocol

    Pages 173-192

    Modeling and Analysis of Repeat RNA Toxicity in Drosophila

  15. No Access

    Book Protocol

    Pages 193-199

    Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis

  16. No Access

    Book Protocol

    Pages 201-217

    Kinetic Analysis of Aggregation Data

  17. No Access

    Book Protocol

    Pages 219-228

    A Bioinformatics Method for Identifying Q/N-Rich Prion-Like Domains in Proteins

  18. No Access

    Book Protocol

    Pages 229-239

    Detecting Soluble PolyQ Oligomers and Investigating Their Impact on Living Cells Using Split-GFP

  19. No Access

    Book Protocol

    Pages 241-253

    Cell Biological Approaches to Investigate Polyglutamine-Expanded AR Metabolism

  20. Back Matter

    Pages 255-258