Volume 1014 2013

Corneal Regenerative Medicine

Methods and Protocols

Editors:

ISBN: 978-1-62703-431-9 (Print) 978-1-62703-432-6 (Online)

Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii

  2. Perspectives

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-43

      Limbal Epithelial Cell Therapy: Past, Present, and Future

    3. No Access

      Book Protocol

      Pages 45-52

      The Artificial Cornea

  3. Corneal Stem Cell Enrichment and Characterization

    1. Front Matter

      Pages 53-53

    2. No Access

      Book Protocol

      Pages 55-64

      Clonal Analysis of Limbal Epithelial Stem Cell Populations

    3. No Access

      Book Protocol

      Pages 65-70

      The Use of Bromodeoxyuridine Incorporation Assays to Assess Corneal Stem Cell Proliferation

    4. No Access

      Book Protocol

      Pages 71-77

      Enrichment of Human Corneal Epithelial Stem/Progenitor Cells by Magnetic Bead Sorting Using SSEA4 as a Negative Marker

    5. No Access

      Book Protocol

      Pages 79-99

      Limbal Epithelial Stem Cell Identification Using Immunoblotting Analysis

  4. Corneal Cell Culture

    1. Front Matter

      Pages 101-101

    2. No Access

      Book Protocol

      Pages 103-129

      The Culture of Limbal Epithelial Cells

    3. No Access

      Book Protocol

      Pages 131-139

      The Culture of Limbal Stromal Cells and Corneal Endothelial Cells

  5. Development of the Artificial Cornea

    1. Front Matter

      Pages 141-141

    2. No Access

      Book Protocol

      Pages 143-155

      The Formation of a Tissue-Engineered Cornea Using Plastically Compressed Collagen Scaffolds and Limbal Stem Cells

    3. No Access

      Book Protocol

      Pages 157-164

      Fabrication of a Human Recombinant Collagen-Based Corneal Substitute Using Carbodiimide Chemistry

    4. No Access

      Book Protocol

      Pages 165-178

      Fabrication of a Corneal-Limbal Tissue Substitute Using Silk Fibroin

    5. No Access

      Book Protocol

      Pages 179-185

      Cultivation of Limbal Epithelial Cells on Electrospun Poly (lactide-co-glycolide) Scaffolds for Delivery to the Cornea

    6. No Access

      Book Protocol

      Pages 187-199

      The Growth and Delivery of Mesenchymal and Limbal Stem Cells Using Copolymer Polyamide 6/12 Nanofiber Scaffolds

    7. No Access

      Book Protocol

      Pages 201-210

      Encapsulation and Culture of Mammalian Cells Including Corneal Cells in Alginate Hydrogels

    8. No Access

      Book Protocol

      Pages 211-223

      In Vivo Confocal Microscopy of the Cornea to Assess Tissue Regenerative Response After Biomaterial Implantation in Humans

  6. Back Matter

    Pages 225-230