Volume 1010 2013

Trinucleotide Repeat Protocols

Editors:

ISBN: 978-1-62703-410-4 (Print) 978-1-62703-411-1 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii

  2. Introduction for Trinucleotide Repeat Diseases

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-17

      A Brief History of Triplet Repeat Diseases

    3. No Access

      Book Protocol

      Pages 19-32

      Huntington’s Disease and Cell Therapies: Past, Present, and Future

  3. Analysis of Synaptic Plasticity

    1. Front Matter

      Pages 33-33

    2. No Access

      Book Protocol

      Pages 35-43

      Transcranial Two-Photon Imaging of Synaptic Structures in the Cortex of Awake Head-Restrained Mice

    3. No Access

      Book Protocol

      Pages 45-57

      In Vivo Imaging of Synapse Plasticity in the Mouse Motor Cortex

  4. Targeting on ES Cells

    1. Front Matter

      Pages 59-59

    2. No Access

      Book Protocol

      Pages 61-77

      Pluripotent Hybrid Stem Cells from Transgenic Huntington’s Disease Monkey

    3. No Access

      Book Protocol

      Pages 79-91

      Mesenchymal Stem Cells for Trinucleotide Repeat Disorders

  5. Targeting on RNA

    1. Front Matter

      Pages 93-93

    2. No Access

      Book Protocol

      Pages 95-109

      Lentiviral-Mediated Gene Transfer of siRNAs for the Treatment of Huntington’s Disease

    3. No Access

      Book Protocol

      Pages 111-121

      Purification of dFMR1-Containing Complexes Using Tandem Affinity Purification

    4. No Access

      Book Protocol

      Pages 123-138

      Combined FISH and Immunofluorescent Staining Methods to Co-localize Proteins and mRNA in Neurons and Brain Tissue

  6. Modification of Proteins and DNAs

    1. Front Matter

      Pages 139-139

    2. No Access

      Book Protocol

      Pages 141-153

      Epigenetic Modifications of the FMR1 Gene

    3. No Access

      Book Protocol

      Pages 155-176

      The CGG Repeat and the FMR1 Gene

    4. No Access

      Book Protocol

      Pages 177-200

      Study of Oxidative Damage and Antioxidant Systems in Two Huntington’s Disease Rodent Models

    5. No Access

      Book Protocol

      Pages 201-209

      Regulation of Ataxin-1 Phosphorylation and Its Impact on Biology

  7. Trinucleotide Repeat Expansion and Detection

    1. Front Matter

      Pages 211-211

    2. No Access

      Book Protocol

      Pages 213-230

      Towards Understanding Region-Specificity of Triplet Repeat Diseases: Coupled Immunohistology and Mass Spectrometry Imaging

    3. No Access

      Book Protocol

      Pages 231-251

      Antibodies and Intrabodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant Forms

    4. No Access

      Book Protocol

      Pages 253-264

      Cell Recovery from DM1 Transgenic Mouse Tissue to Study (CTG) n Instability and DM1 Pathogenesis

    5. No Access

      Book Protocol

      Pages 265-276

      Markerless Modification of Trinucleotide Repeat Loci in BACs

    6. No Access

      Book Protocol

      Pages 277-292

      DRPLA: Recent Advances in Research Using Transgenic Mouse Models

  8. Back Matter

    Pages 293-296