Volume 1005 2013

Heart Proteomics

Methods and Protocols

Editors:

ISBN: 978-1-62703-385-5 (Print) 978-1-62703-386-2 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii

  2. No Access

    Book Protocol

    Pages 1-10

    Large-Scale Characterization of the Murine Cardiac Proteome

  3. No Access

    Book Protocol

    Pages 11-24

    Determining Protein Concentrations of the Human Ventricular Proteome

  4. No Access

    Book Protocol

    Pages 25-38

    Multidimensional Protein Identification Technology for Direct-Tissue Proteomics of Heart

  5. No Access

    Book Protocol

    Pages 39-52

    Global Protein Quantification of Mouse Heart Tissue Based on the SILAC Mouse

  6. No Access

    Book Protocol

    Pages 53-66

    Global Proteomic Profiling and Enrichment Maps of Dilated Cardiomyopathy

  7. No Access

    Book Protocol

    Pages 67-76

    Characterization of the Human Myocardial Proteome in Dilated Cardiomyopathy by Label-Free Quantitative Shotgun Proteomics of Heart Biopsies

  8. No Access

    Book Protocol

    Pages 77-93

    Systems Proteomics of Healthy and Diseased Chromatin

  9. No Access

    Book Protocol

    Pages 95-107

    Proteomic Analysis of Interstitial Aortic Valve Cells Acquiring a Pro-calcific Profile

  10. No Access

    Book Protocol

    Pages 109-117

    Differential Protein Expression Analysis of Degenerative Aortic Stenosis by iTRAQ Labeling

  11. No Access

    Book Protocol

    Pages 119-127

    Characterization of Zebrafish Cardiac Proteome Using Online pH Gradient SCX–RP HPLC–MS/MS Platform

  12. No Access

    Book Protocol

    Pages 129-141

    Proteomic Analysis of Brain Mitochondrial Proteome and Mitochondrial Complexes

  13. No Access

    Book Protocol

    Pages 143-156

    Oxidative Modifications of Mitochondria Complex II

  14. No Access

    Book Protocol

    Pages 157-168

    Detection of O-GlcNAc Modifications on Cardiac Myofilament Proteins

  15. No Access

    Book Protocol

    Pages 169-179

    Quantification of Mitochondrial S-Nitrosylation by CysTMT6 Switch Assay

  16. No Access

    Book Protocol

    Pages 181-197

    Identification of Thioredoxin Target Protein Networks in Cardiac Tissues of a Transgenic Mouse

  17. No Access

    Book Protocol

    Pages 199-213

    Using Pure Protein to Build a Multiple Reaction Monitoring Mass Spectrometry Assay for Targeted Detection and Quantitation

  18. No Access

    Book Protocol

    Pages 215-223

    A Sequential Extraction Methodology for Cardiac Extracellular Matrix Prior to Proteomics Analysis

  19. No Access

    Book Protocol

    Pages 225-235

    Optimized Method for Identification of the Proteomes Secreted by Cardiac Cells

  20. No Access

    Book Protocol

    Pages 237-243

    Secretome of Human Aortic Valves

  21. No Access

    Book Protocol

    Pages 245-256

    A Comparative Study of Immunodepletion and Equalization Methods for Aortic Stenosis Human Plasma

  22. Back Matter

    Pages 257-262