Chemical Neurobiology

Methods and Protocols

Editors:

ISBN: 978-1-62703-344-2 (Print) 978-1-62703-345-9 (Online)

Table of contents (14 protocols)

  1. Front Matter

    Pages i-xii

  2. Chemical Probes of Membrane Protein Structure and Function

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-17

      Engineering K+ Channels Using Semisynthesis

    3. No Access

      Book Protocol

      Pages 19-30

      Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes

    4. No Access

      Book Protocol

      Pages 31-42

      Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules

    5. No Access

      Book Protocol

      Pages 43-54

      A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR Heterodimers

  3. Photochemical Control of Protein and Cellular Function

    1. Front Matter

      Pages 55-55

    2. No Access

      Book Protocol

      Pages 57-77

      Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy

    3. No Access

      Book Protocol

      Pages 79-87

      Characterization of One- and Two-Photon Photochemical Uncaging Efficiency

    4. No Access

      Book Protocol

      Pages 89-105

      Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization

    5. No Access

      Book Protocol

      Pages 107-120

      A 1H NMR Assay for Measuring the Photostationary States of Photoswitchable Ligands

    6. No Access

      Book Protocol

      Pages 121-129

      Developing a Photoreactive Antagonist

  4. Chemical Probes for Imaging in the Nervous System

    1. Front Matter

      Pages 131-131

    2. No Access

      Book Protocol

      Pages 133-145

      Development and In Vitro Characterization of Ratiometric and Intensity-Based Fluorescent Ion Sensors

    3. No Access

      Book Protocol

      Pages 147-160

      Characterization of Voltage-Sensitive Dyes in Living Cells Using Two-Photon Excitation

    4. No Access

      Book Protocol

      Pages 161-178

      Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor

    5. No Access

      Book Protocol

      Pages 179-191

      Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots

    6. No Access

      Book Protocol

      Pages 193-205

      Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI

  5. Back Matter

    Pages 207-219