Cellular and Subcellular Nanotechnology

Methods and Protocols

Editors:

ISBN: 978-1-62703-335-0 (Print) 978-1-62703-336-7 (Online)

Table of contents (32 protocols)

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  1. Front Matter

    Pages i-xvi

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    Pages 1-8

    Nanoparticle-GFP “Chemical Nose” Sensor for Cancer Cell Identification

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    Pages 9-23

    A Method to Map Spatiotemporal pH Changes in a Multicellular Living Organism Using a DNA Nanosensor

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    Pages 25-31

    A Simple Method to Visualize and Assess the Integrity of Lysosomal Membrane in Mammalian Cells Using a Fluorescent Dye

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    Pages 33-39

    Gold Nanoparticle as a Marker for Precise Localization of Nano-objects Within Intracellular Sub-domains

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    Pages 41-46

    Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation

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    Pages 47-55

    Methods for Isolation and Identification of Nanoparticle-Containing Subcellular Compartments

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    Pages 57-63

    Permeabilization of Cell Membrane for Delivery of Nano-objects to Cellular Sub-domains

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    Pages 65-80

    A Method to Encapsulate Molecular Cargo Within DNA Icosahedra

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    Pages 81-91

    Delivery of Plasmid DNA to Mammalian Cells Using Polymer–Gold Nanorod Assemblies

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    Pages 93-98

    Lipophilic-Formulated Gold Porphyrin Nanoparticles for Chemotherapy

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    Pages 99-112

    Mitochondria-Specific Nano-Emulsified Therapy for Myocardial Protection Against Doxorubicin-Induced Cardiotoxicity

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    Pages 113-125

    Formation of Pit-Spanning Phospholipid Bilayers on Nanostructured Silicon Dioxide Surfaces for Studying Biological Membrane Events

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    Pages 127-137

    Characterization of Nanoparticle–Lipid Membrane Interactions Using QCM-D

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    Pages 139-148

    Single-Cell Nanosurgery

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    Pages 149-162

    Single Quantum Dot Imaging in Living Cells

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    Pages 163-169

    Fabrication of Fluorescent Silica Nanoparticles with Aggregation-Induced Emission Luminogens for Cell Imaging

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    Pages 171-184

    Monitoring the Degradation of Reduction-Sensitive Gene Carriers with Fluorescence Spectroscopy and Flow Cytometry

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    Pages 185-193

    Quantification of Intracellular Mitochondrial Displacements in Response to Nanomechanical Forces

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    Pages 195-209

    Imaging Select Mammalian Organelles Using Fluorescent Microscopy: Application to Drug Delivery

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    Pages 211-223

    Real-Time Particle Tracking for Studying Intracellular Trafficking of Pharmaceutical Nanocarriers

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