Plant Meiosis

Methods and Protocols

Editors:

ISBN: 978-1-62703-332-9 (Print) 978-1-62703-333-6 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xv

  2. Cytological Techniques for Light Microscopy

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-11

      Spreading and Fluorescence In Situ Hybridization of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenetical Analysis

    3. No Access

      Book Protocol

      Pages 13-24

      Analysis of Plant Meiotic Chromosomes by Chromosome Painting

    4. No Access

      Book Protocol

      Pages 25-34

      Using Sequential Fluorescence and Genomic In Situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus

    5. No Access

      Book Protocol

      Pages 35-43

      Labeling Meiotic Chromosomes in Maize with Fluorescence In Situ Hybridization

    6. No Access

      Book Protocol

      Pages 45-52

      Examining Female Meiocytes of Maize by Confocal Microscopy

    7. No Access

      Book Protocol

      Pages 53-66

      Three-Dimensional Acrylamide Fluorescence In Situ Hybridization for Plant Cells

    8. No Access

      Book Protocol

      Pages 67-78

      Analyzing Maize Meiotic Chromosomes with Super-Resolution Structured Illumination Microscopy

    9. No Access

      Book Protocol

      Pages 79-92

      Live Imaging of Chromosome Dynamics

    10. No Access

      Book Protocol

      Pages 93-101

      Immunolocalization of Meiotic Proteins in Brassicaceae: Method 1

    11. No Access

      Book Protocol

      Pages 103-107

      Immunolocalization of Meiotic Proteins in Arabidopsis thaliana: Method 2

    12. No Access

      Book Protocol

      Pages 109-118

      Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana: Method 3

    13. No Access

      Book Protocol

      Pages 119-123

      A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana

    14. No Access

      Book Protocol

      Pages 125-134

      Analyzing Meiotic Chromosomes in Rice

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      Book Protocol

      Pages 135-144

      Analyzing Meiosis in Barley

  3. Cytological Techniques for Electron Microscopy

    1. Front Matter

      Pages 145-145

    2. No Access

      Book Protocol

      Pages 147-158

      Preparing SC Spreads with RNs for EM Analysis

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      Book Protocol

      Pages 159-166

      Analysis of the Synaptonemal Complex in Brassica Using TEM

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      Book Protocol

      Pages 167-174

      Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy

  4. Genetics and Molecular Biology Techniques

    1. Front Matter

      Pages 175-175

    2. No Access

      Book Protocol

      Pages 177-190

      Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen

    3. No Access

      Book Protocol

      Pages 191-201

      Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize

    4. No Access

      Book Protocol

      Pages 203-213

      Analyzing the Meiotic Transcriptome Using Isolated Meiocytes of Arabidopsis thaliana

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