Skin Stem Cells

Methods and Protocols

Editors:

ISBN: 978-1-62703-329-9 (Print) 978-1-62703-330-5 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-9

    Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin

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    Pages 11-19

    Isolation and Cultivation of Human Scalp Interfollicular Epidermal Stem Cells

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    Pages 21-32

    Isolation of Hair Follicle Bulge Stem Cells from YFP-Expressing Reporter Mice

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    Pages 33-43

    Analysis of Bulge Stem Cells from the Epidermis Using Flow Cytometry

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    Pages 45-60

    Lineage Tracing of Hair Follicle Stem Cells in Epidermal Whole Mounts

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    Pages 61-69

    Isolation and Characterization of Cutaneous Epithelial Stem Cells

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    Pages 71-81

    Identification and Analysis of Epidermal Stem Cells from Primary Mouse Keratinocytes

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    Pages 83-97

    Monitoring the Cycling Activity of Cultured Human Keratinocytes Using a CFSE-Based Dye Tracking Approach

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    Pages 99-108

    Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells

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    Pages 109-117

    Analysis of Gene Expression in Skin Using Laser Capture Microdissection

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    Pages 119-142

    Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to Evaluate the Reliability of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction

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    Pages 143-151

    Genetic Manipulation of Keratinocyte Stem Cells with Lentiviral Vectors

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    Pages 153-164

    Using 3D Culture to Investigate the Role of Mechanical Signaling in Keratinocyte Stem Cells

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    Pages 165-182

    In Vivo Transplantation Assay at Limiting Dilution to Identify the Intrinsic Tissue Reconstitutive Capacity of Keratinocyte Stem Cells and Their Progeny

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    Pages 183-192

    Melanoblasts as Multipotent Cells in Murine Skin

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    Pages 193-215

    Generation of Human Melanocytes from Induced Pluripotent Stem Cells

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    Pages 217-233

    Cellular Populations Isolated from Newborn Mouse Skin Including Mesenchymal Stem Cells

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    Pages 235-246

    Isolation, Characterization, and Differentiation of Human Multipotent Dermal Stem Cells

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    Pages 247-263

    Isolation and Differentiation of Hair Follicle-Derived Dermal Precursors

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    Pages 265-274

    Isolation of Mesenchymal Stem Cells from Human Dermis

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