Protein Acetylation

Methods and Protocols

Editors:

ISBN: 978-1-62703-304-6 (Print) 978-1-62703-305-3 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-11

    Validation of Protein Acetylation by Mass Spectrometry

  3. No Access

    Book Protocol

    Pages 13-23

    Application of the CIRAD Mass Spectrometry Approach for Lysine Acetylation Site Discovery

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    Book Protocol

    Pages 25-36

    Application of the MIDAS Approach for Analysis of Lysine Acetylation Sites

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    Book Protocol

    Pages 37-45

    Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein Acetylations

  6. No Access

    Book Protocol

    Pages 47-56

    Towards the N-Terminal Acetylome: An N-Terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin

  7. No Access

    Book Protocol

    Pages 57-93

    Identification and Analysis of O-Acetylated Sialoglycoproteins

  8. No Access

    Book Protocol

    Pages 95-102

    HPLC-Based Quantification of In Vitro N-Terminal Acetylation

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    Book Protocol

    Pages 103-113

    Separation and Purification of Multiply Acetylated Proteins Using Cation-Exchange Chromatography

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    Book Protocol

    Pages 115-126

    In-Gel N-Acetylation for the Quantification of the Degree of Protein In Vivo N-Terminal Acetylation

  11. No Access

    Book Protocol

    Pages 127-136

    Computational Prediction of Lysine Acetylation Proteome-Wide

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    Book Protocol

    Pages 137-150

    Generation and Characterization of Pan-Specific Anti-acetyllysine Antibody

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    Book Protocol

    Pages 151-165

    Using Functional Proteome Microarrays to Study Protein Lysine Acetylation

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    Book Protocol

    Pages 167-176

    Quantitation of Nucleosome Acetylation and Other Histone Posttranslational Modifications Using Microscale NU-ELISA

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    Book Protocol

    Pages 177-192

    Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core

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    Book Protocol

    Pages 193-200

    Production of Amino-Terminally Acetylated Recombinant Proteins in E. coli

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    Book Protocol

    Pages 201-210

    Identification of Lysine Acetyltransferase Substrates Using Bioorthogonal Chemical Proteomics

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    Book Protocol

    Pages 211-227

    Nonradioactive In Vitro Assays for Histone Deacetylases

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    Book Protocol

    Pages 229-238

    The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes

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    Book Protocol

    Pages 239-261

    Analysis of Protein Acetyltransferase Structure–Function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators

  21. Back Matter

    Pages 263-266