Dictyostelium discoideum Protocols

Editors:

ISBN: 978-1-62703-301-5 (Print) 978-1-62703-302-2 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xii

  2. Book Protocol

    Pages 1-15

    The Amoebozoa

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    Book Protocol

    Pages 17-37

    The Model Organism Dictyostelium discoideum

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    Book Protocol

    Pages 39-58

    Comparative Genomics of the Dictyostelids

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    Book Protocol

    Pages 59-92

    One Stop Shop for Everything Dictyostelium: dictyBase and the Dicty Stock Center in 2012

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    Book Protocol

    Pages 93-112

    Fluorescent Reporters and Methods to Analyze Fluorescent Signals

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    Book Protocol

    Pages 113-124

    Collection and Cultivation of Dictyostelids from the Wild

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    Book Protocol

    Pages 125-138

    Identification and Verification of microRNAs by High-Throughput Sequencing

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    Book Protocol

    Pages 139-171

    Transcriptional Profiling of Dictyostelium with RNA Sequencing

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    Book Protocol

    Pages 173-183

    Analysis of Chromatin Organization by Deep Sequencing Technologies

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    Book Protocol

    Pages 185-204

    Pharmacogenetics of Resistance to Cisplatin and Other Anticancer Drugs and the Role of Sphingolipid Metabolism

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    Book Protocol

    Pages 205-229

    N-Glycomic and N-Glycoproteomic Studies in the Social Amoebae

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    Book Protocol

    Pages 231-248

    Measuring Cheating, Fitness, and Segregation in Dictyostelium discoideum

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    Book Protocol

    Pages 249-267

    The Application of the Cre-loxP System for Generating Multiple Knock-out and Knock-in Targeted Loci

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    Book Protocol

    Pages 269-281

    Extrachromosomal Inducible Expression

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    Book Protocol

    Pages 283-294

    Isolation of Dictyostelium Nuclei for Light and Electron Microscopy

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    Book Protocol

    Pages 295-310

    Investigation of DNA Repair Pathway Activity

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    Book Protocol

    Pages 311-323

    Transcript Localization in Dictyostelium discoideum Cells by RNA FISH

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    Book Protocol

    Pages 325-344

    Analysis of Mitochondrial Gene Expression

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    Book Protocol

    Pages 345-366

    Mitochondrial Respiratory Complex Function and the Phenotypic Consequences of Dysfunction

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    Book Protocol

    Pages 367-382

    Micropipette Aspiration for Studying Cellular Mechanosensory Responses and Mechanics

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