Immune Homeostasis

Methods and Protocols

Editors:

ISBN: 978-1-62703-289-6 (Print) 978-1-62703-290-2 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-14

    Quantitating Lymphocyte Programmed Cell Death In Vitro Using Simple Kill Assays

  3. No Access

    Book Protocol

    Pages 15-23

    Fluorescence-Activated Cell Sorting-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death in Activated, Primary Human T Cells

  4. No Access

    Book Protocol

    Pages 25-31

    Evaluation of IL-2-Withdrawal-Induced Apoptosis in Human T Lymphocytes

  5. No Access

    Book Protocol

    Pages 33-41

    Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multiparameter Fluorescence-Activated Cell Sorting-Based Technique

  6. No Access

    Book Protocol

    Pages 43-49

    Visualization of Fas-Mediated Death-Inducing Signaling Complex Formation by Immunoprecipitation

  7. No Access

    Book Protocol

    Pages 51-63

    Analyses of Programmed Cell Death in Dendritic Cells

  8. No Access

    Book Protocol

    Pages 65-70

    Detection of Necrosis by Release of Lactate Dehydrogenase Activity

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    Book Protocol

    Pages 71-79

    Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I and II Tetramers

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    Book Protocol

    Pages 81-106

    Homeostatic Proliferation of Mature T Cells

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    Book Protocol

    Pages 107-131

    Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers

  12. No Access

    Book Protocol

    Pages 133-145

    Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis

  13. No Access

    Book Protocol

    Pages 147-159

    Molecular Measurement of T Cell Receptor Excision Circles

  14. No Access

    Book Protocol

    Pages 161-173

    Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis

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    Book Protocol

    Pages 175-187

    Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders

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    Book Protocol

    Pages 189-197

    Flow Cytometric Measurement of SLAM-Associated Protein and X-Linked Inhibitor of Apoptosis

  17. No Access

    Book Protocol

    Pages 199-212

    In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells

  18. Back Matter

    Pages 213-215