Pancreatic Cancer

Methods and Protocols

Editors:

ISBN: 978-1-62703-286-5 (Print) 978-1-62703-287-2 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-12

    Identification and Analysis of Precursors to Invasive Pancreatic Cancer

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    Pages 13-28

    Tissue Microarrays: Construction and Use

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    Pages 29-42

    Immunohistochemistry of Pancreatic Neoplasia

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    Pages 43-59

    A Method for Conducting Highly Sensitive MicroRNA In Situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer

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    Pages 61-120

    Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications

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    Pages 121-129

    Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis

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    Pages 131-156

    DNA Methylation Analysis in Human Cancer

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    Pages 157-173

    Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients

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    Pages 175-203

    Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine

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    Pages 205-214

    The Prevention and Genetics of Pancreatic Cancer: A Programmatic Approach

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    Pages 215-223

    Development of Orthotopic Pancreatic Tumor Mouse Models

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    Pages 225-248

    Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems

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    Pages 249-266

    Quantification of Murine Pancreatic Tumors by High-Resolution Ultrasound

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    Pages 267-279

    Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells

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    Pages 281-290

    Sphere-Forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells

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    Pages 291-300

    Analysis of Transplanted Bone Marrow-Derived Cells in Chronic Pancreatitis

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    Pages 301-319

    Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo

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    Pages 321-329

    The Use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells

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    Pages 331-340

    Analysis of Tumor-Associated Mucin Glycotopes by Western Transfer Methods

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    Pages 341-351

    Evaluating Tumor Angiogenesis

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