Lipid-Protein Interactions

Methods and Protocols

Editors:

ISBN: 978-1-62703-274-2 (Print) 978-1-62703-275-9 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Book Protocol

    Pages 1-21

    Quartz Crystal Microbalances as Tools for Probing Protein–Membrane Interactions

  3. No Access

    Book Protocol

    Pages 23-36

    Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipid Membranes

  4. No Access

    Book Protocol

    Pages 37-53

    Probing the Thermodynamics of Protein–Lipid Interactions by Isothermal Titration Calorimetry

  5. No Access

    Book Protocol

    Pages 55-71

    Differential Scanning Calorimetry of Protein–Lipid Interactions

  6. No Access

    Book Protocol

    Pages 73-110

    Atomic Force Microscopy and Spectroscopy to Probe Single Membrane Proteins in Lipid Bilayers

  7. No Access

    Book Protocol

    Pages 111-118

    Optimized Negative-Staining Protocol for Examining Lipid-Protein Interactions by Electron Microscopy

  8. No Access

    Book Protocol

    Pages 119-150

    Examining Protein–Lipid Complexes Using Neutron Scattering

  9. No Access

    Book Protocol

    Pages 151-176

    Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment

  10. No Access

    Book Protocol

    Pages 177-218

    Structural Characterization of Membrane Proteins and Peptides by FTIR and ATR-FTIR Spectroscopy

  11. No Access

    Book Protocol

    Pages 219-232

    Förster Resonance Energy Transfer as a Tool for Quantification of Protein–Lipid Selectivity

  12. No Access

    Book Protocol

    Pages 233-252

    A Guide to Tracking Single Transmembrane Proteins in Supported Lipid Bilayers

  13. No Access

    Book Protocol

    Pages 253-278

    Fluorescence Correlation Spectroscopy to Examine Protein–Lipid Interactions in Membranes

  14. No Access

    Book Protocol

    Pages 279-295

    Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching

  15. No Access

    Book Protocol

    Pages 297-328

    Studying Lipid–Protein Interactions with Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids

  16. No Access

    Book Protocol

    Pages 329-355

    EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid Bilayers

  17. No Access

    Book Protocol

    Pages 357-387

    Solid-State NMR Approaches to Study Protein Structure and Protein–Lipid Interactions

  18. No Access

    Book Protocol

    Pages 389-413

    Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in a Lipid Environment

  19. No Access

    Book Protocol

    Pages 415-433

    Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

  20. No Access

    Book Protocol

    Pages 435-455

    The Simulation Approach to Lipid–Protein Interactions

  21. Back Matter

    Pages 457-464