Dopamine

Methods and Protocols

Editors:

ISBN: 978-1-62703-250-6 (Print) 978-1-62703-251-3 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi

  2. Biochemical, Proteomic, and Computational Tools

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-13

      Detection of Cell Surface Dopamine Receptors

    3. No Access

      Book Protocol

      Pages 15-24

      Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells

    4. No Access

      Book Protocol

      Pages 25-42

      Methods of Dopamine Research in Retina Cells

    5. No Access

      Book Protocol

      Pages 43-60

      Capture of D2 Dopamine Receptor Signaling Complexes in Striatal Cells for Mass Spectrometry Proteomic Analysis

    6. No Access

      Book Protocol

      Pages 61-75

      Modeling Spatial Aspects of Intracellular Dopamine Signaling

  3. Cellular Imaging

    1. Front Matter

      Pages 77-77

    2. No Access

      Book Protocol

      Pages 79-94

      A Biophysical Approach for the Study of Dopamine Receptor Oligomerization

    3. No Access

      Book Protocol

      Pages 95-105

      Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET Technology

    4. No Access

      Book Protocol

      Pages 107-122

      BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling

    5. No Access

      Book Protocol

      Pages 123-138

      Dopaminergic Regulation of Dendritic Calcium: Fast Multisite Calcium Imaging

  4. Genetic Manipulation in Cells and Organisms

    1. Front Matter

      Pages 139-139

    2. No Access

      Book Protocol

      Pages 141-180

      Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosolic End of Transmembrane Region 6

    3. No Access

      Book Protocol

      Pages 181-200

      A Molecular Genetic Approach to Uncovering the Differential Functions of Dopamine D2 Receptor Isoforms

    4. No Access

      Book Protocol

      Pages 201-214

      Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish

    5. No Access

      Book Protocol

      Pages 215-225

      Application of Cell-Specific Isolation to the Study of Dopamine Signaling in Drosophila

  5. Electrochemical, Physiological, and Behavioral Analysis

    1. Front Matter

      Pages 227-227

    2. No Access

      Book Protocol

      Pages 229-242

      Regulation of Dopamine Transporter Expression by Neuronal Activity

    3. No Access

      Book Protocol

      Pages 243-273

      Monitoring Axonal and Somatodendritic Dopamine Release Using Fast-Scan Cyclic Voltammetry in Brain Slices

    4. No Access

      Book Protocol

      Pages 275-294

      Real-Time Chemical Measurements of Dopamine Release in the Brain

    5. No Access

      Book Protocol

      Pages 295-308

      The MPTP/Probenecid Model of Progressive Parkinson’s Disease

  6. Back Matter

    Pages 309-312