Bacterial Cell Surfaces

Methods and Protocols

Editors:

ISBN: 978-1-62703-244-5 (Print) 978-1-62703-245-2 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-14

    A Bird’s Eye View of the Bacterial Landscape

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    Pages 15-35

    Visualizing the Bacterial Cell Surface: An Overview

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    Pages 37-52

    Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems

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    Pages 53-75

    Experimental Manipulation of the Microbial Functional Amyloid Called Curli

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    Pages 77-95

    Visualization of Gram-positive Bacterial Pili

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    Pages 97-107

    Single Cell Microfluidic Studies of Bacterial Motility

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    Pages 109-120

    Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule

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    Pages 121-131

    Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy

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    Pages 133-155

    Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance

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    Pages 157-172

    Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System

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    Pages 173-185

    Pore Formation by T3SS Translocators: Liposome Leakage Assay

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    Pages 187-204

    Isolation of Bacterial Type IV Machine Subassemblies

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    Pages 205-222

    Production and Crystallization of Bacterial Type V Secretion Proteins

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    Pages 223-237

    Assembly of Bacterial Outer Membrane Proteins

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    Pages 239-258

    The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization

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    Pages 259-272

    Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles

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    Pages 273-288

    In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate

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    Pages 289-308

    Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes

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    Pages 309-324

    Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis

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    Pages 325-336

    Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine Residues

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