Cell Senescence

Methods and Protocols

Editors:

ISBN: 978-1-62703-238-4 (Print) 978-1-62703-239-1 (Online)

Table of contents (34 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-13

    Cell Senescence as Both a Dynamic and a Static Phenotype

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    Pages 15-35

    Senescence Regulation by mTOR

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    Pages 37-61

    Senescence Regulation by the p53 Protein Family

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    Pages 63-81

    Markers of Cellular Senescence

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    Pages 83-92

    Biomarkers of Cell Senescence Assessed by Imaging Cytometry

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    Pages 93-120

    Cytofluorometric Assessment of Cell Cycle Progression

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    Pages 121-142

    Quantification of Cell Cycle-Arresting Proteins

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    Pages 143-156

    Colorimetric Detection of Senescence-Associated β Galactosidase

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    Pages 157-163

    Chemiluminescent Detection of Senescence-Associated β Galactosidase

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    Pages 165-173

    Detection of the Senescence-Associated Secretory Phenotype (SASP)

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    Pages 175-184

    Unbiased Characterization of the Senescence-Associated Secretome Using SILAC-Based Quantitative Proteomics

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    Pages 185-196

    Detection of Senescence-Associated Heterochromatin Foci (SAHF)

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    Pages 197-213

    Monitoring DNA Damage During Cell Senescence

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    Pages 215-231

    Assessment and Quantification of Telomerase Enzyme Activity

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    Pages 233-242

    Methods for the Assessment of Telomere Status

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    Pages 243-251

    Detection of Nuclear Envelope Alterations in Senescence

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    Pages 253-263

    Measuring Reactive Oxygen Species in Senescent Cells

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    Pages 265-281

    Quantification of Protein Carbonylation

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    Pages 283-296

    Assays for the Measurement of Lipid Peroxidation

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    Pages 297-312

    Raman Spectroscopy for the Detection of AGEs/ALEs

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