p53 Protocols

Editors:

ISBN: 978-1-62703-235-3 (Print) 978-1-62703-236-0 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Book Protocol

    Pages 1-14

    Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues

  3. No Access

    Book Protocol

    Pages 15-29

    Detecting p53 Isoforms at Protein Level

  4. No Access

    Book Protocol

    Pages 31-48

    Autophagy, Senescence, and Apoptosis

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    Book Protocol

    Pages 49-61

    p53 and Cell Cycle Effects After DNA Damage

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    Book Protocol

    Pages 63-73

    p53 Ubiquitination and Proteasomal Degradation

  7. No Access

    Book Protocol

    Pages 75-84

    Identification of p53 in Mitochondria

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    Book Protocol

    Pages 85-94

    Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis

  9. No Access

    Book Protocol

    Pages 95-111

    Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization

  10. No Access

    Book Protocol

    Pages 113-125

    Determine the Effect of p53 on Chemosensitivity

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    Pages 127-133

    Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells

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    Pages 135-146

    Mutant p53 in Cell Adhesion and Motility

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    Pages 147-155

    Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication

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    Pages 157-164

    Generation of p53-Deficient Induced Pluripotent Stem Cells from Mouse Embryo Fibroblasts

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    Pages 165-181

    p53 Actions on MicroRNA Expression and Maturation Pathway

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    Pages 183-191

    Isolation and Characterization of Murine Multipotent Lung Stem Cells

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    Pages 193-199

    Generation of p53 Knock-down Cell Lines

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    Pages 201-210

    ChIP for Identification of p53 Responsive DNA Promoters

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    Pages 211-226

    ChIP-on-chip to Identify Mutant p53 Targets

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    Pages 227-236

    ChIP Sequencing to Identify p53 Targets

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    Book Protocol

    Pages E1-E1

    Erratum to: Chapter 4 P53 and Cell Cycle Effects After DNA Damage

  22. Back Matter

    Pages 237-238