Molecular Dermatology

Methods and Protocols

Editors:

ISBN: 978-1-62703-226-1 (Print) 978-1-62703-227-8 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-16

    Molecular Dermatology Comes of Age

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    Pages 17-32

    Molecular Diagnosis of Autoimmune Blistering Diseases

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    Pages 33-96

    Molecular Diagnosis of Genodermatoses

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    Pages 97-117

    Three-Dimensional Visualization of the Molecular Architecture of Cell–Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections

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    Pages 119-127

    3D Visualization of Epidermal Langerhans Cells

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    Pages 129-140

    In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography

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    Pages 141-150

    Optical Imaging of HPV Infection in a Murine Model

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    Pages 151-164

    Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration

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    Pages 165-177

    Analysis of Cutaneous Somatic Mosaicism

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    Pages 179-191

    Global Proteome Analyses of SILAC-Labeled Skin Cells

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    Pages 193-200

    MicroRNA Profiling During Human Keratinocyte Differentiation Using a Quantitative Real-Time PCR Method

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    Pages 201-218

    Cell Death in the Skin: How to Study Its Quality and Quantity?

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    Pages 219-225

    In Vitro Pathogenicity Assay for Anti-desmoglein Autoantibodies in Pemphigus

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    Pages 227-242

    Induction of Granulocyte-Dependent Dermal-Epidermal Separation by Autoantibodies Ex Vivo

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    Pages 243-252

    Analysis of Collective Invasion of Carcinoma Cells in a 3D Organotypic Model

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    Pages 253-259

    Isolation of Melanoma Tumor-Initiating Cells from Surgical Tissues

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    Pages 261-277

    Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors

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    Pages 279-286

    Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein “Kaede”-Transgenic Mice

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    Pages 287-303

    Reconstitution of Skin Fibrosis Development Using a Tissue Engineering Approach

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    Pages 305-323

    Bioengineered Skin Humanized Model of Psoriasis

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