Transgenic Cotton

Methods and Protocols

Editors:

ISBN: 978-1-62703-211-7 (Print) 978-1-62703-212-4 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-x

  2. Introduction

    1. Front Matter

      Pages 1-1

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      Book Protocol

      Pages 3-15

      Transgenic Cotton: From Biotransformation Methods to Agricultural Application

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      Pages 17-28

      Genetically Modified Cotton in India and Detection Strategies

  3. Transformation

    1. Front Matter

      Pages 29-29

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      Book Protocol

      Pages 31-45

      Agrobacterium-Mediated Transformation of Cotton

    3. No Access

      Book Protocol

      Pages 47-57

      Biolistic Transformation of Cotton Zygotic Embryo Meristem

    4. No Access

      Book Protocol

      Pages 59-70

      Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures

    5. No Access

      Book Protocol

      Pages 71-77

      Cotton Transformation via Pollen Tube Pathway

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      Pages 79-92

      Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.)

  4. Detection

    1. Front Matter

      Pages 93-93

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      Pages 95-107

      Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome

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      Pages 109-130

      Estimating the Copy Number of Transgenes in Transformed Cotton by Real-Time Quantitative PCR

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      Pages 131-138

      Development of Enzyme-Linked Immunosorbent Assay for the Detection of Bt Protein in Transgenic Cotton

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      Pages 139-151

      DNA-Based Diagnostics for Genetically Modified Cotton: Decaplex PCR Assay to Differentiate MON531 and MON15985 Bt Cotton Events

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      Pages 153-160

      A Simple and Rapid Method for Determining Transgenic Cotton Plants

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      Pages 161-164

      An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants

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      Pages 165-176

      Inheritance of Transgenes in Transgenic Bt Lines Resistance to Helicoerpa armigera in Upland Cotton

  5. Application

    1. Front Matter

      Pages 177-177

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      Book Protocol

      Pages 179-187

      Agrobacterium rhizogenes-Induced Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics

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      Book Protocol

      Pages 189-197

      Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation

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      Book Protocol

      Pages 199-210

      Development of Transgenic CryIA(c) + GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover

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      Book Protocol

      Pages 211-222

      Agrobacterium-Mediated Transformation of Cotton (Gossypium hirsutum) Shoot Apex with a Fungal Phytase Gene Improves Phosphorus Acquisition

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      Book Protocol

      Pages 223-246

      Genetic Transformation of Cotton with a Harpin-Encoding Gene hpaXoo Confers an Enhanced Defense Response Against Verticillium dahliae Kleb

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      Book Protocol

      Pages 247-258

      Development of Insect-Resistant Transgenic Cotton with Chimeric TVip3A Accumulating in Chloroplasts

  6. Back Matter

    Pages 273-277

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