Mammalian Oocyte Regulation

Methods and Protocols

Editors:

ISBN: 978-1-62703-190-5 (Print) 978-1-62703-191-2 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-18

    Studying Recombination in Mouse Oocytes

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    Pages 19-45

    Analysis of Recombinants in Female Mouse Meiosis

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    Pages 47-57

    Studying Meiosis-Specific Cohesins in Mouse Embryonic Oocytes

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    Pages 59-75

    Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development

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    Pages 77-89

    Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos

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    Pages 91-106

    Time-Lapse Epifluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes

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    Pages 107-120

    Using FRET to Study RanGTP Gradients in Live Mouse Oocytes

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    Pages 121-134

    Making cRNA for Microinjection and Expression of Fluorescently Tagged Proteins for Live-Cell Imaging in Oocytes

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    Pages 135-151

    RNAi-Based Methods for Gene Silencing in Mouse Oocytes

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    Pages 153-162

    Micro-injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes

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    Pages 163-178

    Measuring Transport and Accumulation of Radiolabeled Substrates in Oocytes and Embryos

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    Pages 179-187

    Immunofluorescence Staining of Spindles, Chromosomes, and Kinetochores in Human Oocytes

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    Pages 189-202

    Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes

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    Pages 203-212

    Chromosome Spreads with Centromere Staining in Mouse Oocytes

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    Pages 213-230

    Preparation of Mammalian Oocytes for Transmission Electron Microscopy

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    Pages 231-248

    Measuring Ca2+ Oscillations in Mammalian Eggs

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    Pages 249-253

    Counting Chromosomes in Intact Eggs

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    Pages 255-265

    Free-Hand Bisection of Mouse Oocytes and Embryos

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    Pages 267-283

    Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes

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    Pages 285-300

    Nuclear Transfer in the Mouse Oocyte

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