Electron Crystallography of Soluble and Membrane Proteins

Methods and Protocols

Editors:

ISBN: 978-1-62703-175-2 (Print) 978-1-62703-176-9 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-16

    Introduction to Electron Crystallography

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    Pages 17-30

    Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis

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    Pages 31-58

    Two-Dimensional Crystallization of Membrane Proteins by Reconstitution Through Dialysis

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    Pages 59-71

    Monolayer Two-Dimensional Crystallization of Membrane Proteins

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    Pages 73-101

    Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples

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    Pages 103-118

    Low Dose Techniques and Cryo-Electron Microscopy

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    Pages 119-128

    Grid Preparation for Cryo-Electron Microscopy

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    Pages 129-152

    Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins

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    Pages 153-169

    The Collection of High-Resolution Electron Diffraction Data

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    Pages 171-194

    Image Processing of 2D Crystal Images

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    Pages 195-209

    Merging of Image Data in Electron Crystallography

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    Pages 211-227

    Evaluation of Electron Crystallographic Data from Images of Two-Dimensional Crystals

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    Pages 229-241

    Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy

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    Pages 243-272

    Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement

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    Pages 273-296

    High-Throughput Methods for Electron Crystallography

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    Pages 297-305

    Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening

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    Pages 307-312

    Automation of Data Acquisition in Electron Crystallography

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    Pages 313-330

    Automation of Image Processing in Electron Crystallography

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    Pages 331-351

    Choice and Maintenance of Equipment for Electron Crystallography

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    Pages 353-379

    Future Developments in Instrumentation for Electron Crystallography

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