Legionella

Methods and Protocols

Editors:

ISBN: 978-1-62703-160-8 (Print) 978-1-62703-161-5 (Online)

Table of contents (37 protocols)

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  1. Front Matter

    Pages i-xxviii

  2. Identification and Typing of Legionella in the Environment and During Disease

    1. Front Matter

      Pages 1-1

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      Pages 3-25

      Identification of Legionella in the Environment

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      Pages 27-56

      Identification of Legionella in Clinical Samples

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      Pages 57-72

      Sequence-Based Identification of Legionella

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      Pages 73-86

      Investigation of Outbreaks: Epidemiology

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      Pages 87-118

      Outbreak Investigations and Identification of Legionella in Contaminated Water

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      Pages 119-148

      Typing Methods for Legionella

  3. Physiology and genetics of Legionella

    1. Front Matter

      Pages 149-149

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      Pages 151-162

      Culturing, Media, and Handling of Legionella

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      Pages 163-181

      The Intracellular Metabolism of Legionella by Isotopologue Profiling

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      Pages 183-195

      Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction

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      Pages 197-212

      Constructing Unmarked Gene Deletions in Legionella pneumophila

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      Pages 213-217

      Natural Biofilm Formation with Legionella pneumophila

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      Pages 219-224

      Production of L. pneumophila Monospecies Biofilms in a Low-Nutrient-Concentration Medium

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      Pages 225-230

      Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila

  4. Cellular microbiology of Legionella

    1. Front Matter

      Pages 231-231

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      Pages 233-249

      Analysis of Legionella Infection by Flow Cytometry

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      Pages 251-264

      Analysis of Legionella Infection Using RNAi in Drosophila Cells

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      Pages 265-277

      Immunofluorescence Imaging of Legionella Infection In Vitro

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      Pages 279-307

      Electron Microscopy of Legionella and Legionella-Infected Cells

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      Pages 309-321

      Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages

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      Pages 323-332

      Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System

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      Pages 333-344

      Subcellular Localization of Legionella Dot/Icm Effectors

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