Peroxisome Proliferator-Activated Receptors (PPARs)

Methods and Protocols

Editors:

ISBN: 978-1-62703-154-7 (Print) 978-1-62703-155-4 (Online)

Table of contents (25 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-6

    PPARs: History and Advances

  3. No Access

    Book Protocol

    Pages 7-34

    PPAR-Alpha Cloning, Expression, and Characterization

  4. No Access

    Book Protocol

    Pages 35-55

    PPARGC1A and PPARG Genotyping: Beginner’s Guide to Genotyping with Unlabeled Probes

  5. No Access

    Book Protocol

    Pages 57-65

    Generation of an Inducible, Cardiomyocyte-Specific Transgenic Mouse Model with PPAR β/δ Overexpression

  6. No Access

    Book Protocol

    Pages 67-86

    Specific Knockdown of PPARδ Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering

  7. No Access

    Book Protocol

    Pages 87-98

    Dominant-Negative and Knockdown Approaches to Studying PPAR Activity

  8. No Access

    Book Protocol

    Pages 99-116

    Producing PPARgamma2 Knockdown in Mouse Liver

  9. No Access

    Book Protocol

    Pages 117-135

    Adipose Tissue-Specific PPARγ Gene Targeting

  10. No Access

    Book Protocol

    Pages 137-144

    Site-Directed Mutagenesis to Study the Role of Specific Amino Acids in the Ligand Binding Domain of PPARs

  11. No Access

    Book Protocol

    Pages 145-161

    PPAR SUMOylation: Some Useful Experimental Tips

  12. No Access

    Book Protocol

    Pages 163-173

    Analyzing Phosphorylation-Dependent Regulation of Subcellular Localization and Transcriptional Activity of Transcriptional Coactivator NT-PGC-1α

  13. No Access

    Book Protocol

    Pages 175-185

    In Vivo Studies of PPAR-Chromatin Interactions: Chromatin Immunoprecipitation for Single-Locus and Genomewide Analyses

  14. No Access

    Book Protocol

    Pages 187-196

    FISH Analysis Using PPAR γ-Specific Probes for Detection of PAX8-PPAR γ Translocation in Follicular Thyroid Neoplasms

  15. No Access

    Book Protocol

    Pages 197-206

    Immunohistochemical Techniques to Identify and Localize Proteins of Interest in Paraffin Embedded Tissue Sections

  16. No Access

    Book Protocol

    Pages 207-217

    Determination of PPAR Expression by Western Blot

  17. No Access

    Book Protocol

    Pages 219-227

    Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators

  18. No Access

    Book Protocol

    Pages 229-235

    Estimation of the PPARα Agonism of Fibrates by a Combined MM-Docking Approach

  19. No Access

    Book Protocol

    Pages 237-252

    Combined Biophysical and Cell-Based Approaches for the Assessment of Ligand Binding to PPARγ

  20. No Access

    Book Protocol

    Pages 253-273

    Exploring PPAR Modulation in Experimental Mice

  21. No Access

    Book Protocol

    Pages 275-286

    Induction of Adipogenic Differentiation in Three-Dimensional Culture Model on a Novel Microfabricated Scaffold

previous Page of 2