Nanoimaging

Methods and Protocols

Editors:

ISBN: 978-1-62703-136-3 (Print) 978-1-62703-137-0 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xiv

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    Pages 1-10

    Introduction: Nanoimaging Techniques in Biology

  3. Light Microscopy

    1. Front Matter

      Pages 11-11

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      Pages 13-26

      Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy

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      Pages 27-41

      4Pi Microscopy

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      Pages 43-64

      Fluorescence In Situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy

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      Pages 65-80

      Two-Color STED Imaging of Synapses in Living Brain Slices

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      Pages 81-93

      Super-Resolution Imaging by Localization Microscopy

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      Pages 95-110

      High-Content Super-Resolution Imaging of Live Cell by uPAINT

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      Pages 111-129

      Super-Resolution Fluorescence Imaging with Blink Microscopy

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      Pages 131-151

      Photoswitchable Fluorophores for Single-Molecule Localization Microscopy

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      Pages 153-167

      Single-Molecule Tracking of mRNA in Living Cells

  4. Electron Microscopy

    1. Front Matter

      Pages 169-169

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      Pages 171-193

      Semiautomatic, High-Throughput, High-Resolution Protocol for Three-Dimensional Reconstruction of Single Particles in Electron Microscopy

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      Pages 195-207

      Mass Mapping of Amyloid Fibrils in the Electron Microscope Using STEM Imaging

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      Pages 209-226

      Elemental Mapping by Electron Energy Loss Spectroscopy in Biology

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      Pages 227-251

      Cellular Nanoimaging by Cryo Electron Tomography

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      Pages 253-273

      Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by SEM-Based Scanning Transmission Electron Microscopy

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      Pages 275-292

      3D Imaging of Cells and Tissues by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM)

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      Pages 293-311

      Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy

  5. Scanning Probe Microscopy

    1. Front Matter

      Pages 313-313

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      Pages 315-341

      Atomic Force Microscopy Imaging of Macromolecular Complexes

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      Pages 343-357

      Imaging of Transmembrane Proteins Directly Incorporated Within Supported Lipid Bilayers Using Atomic Force Microscopy

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      Pages 359-371

      Functional AFM Imaging of Cellular Membranes Using Functionalized Tips

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