Microfluidic Diagnostics

Methods and Protocols

Editors:

ISBN: 978-1-62703-133-2 (Print) 978-1-62703-134-9 (Online)

Table of contents (32 protocols)

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  1. Front Matter

    Pages i-xiii

  2. Microfluidic Diagnostics: From the Classroom to the Boardroom

    1. Front Matter

      Pages 1-1

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      Pages 3-23

      Present Technology and Future Trends in Point-of-Care Microfluidic Diagnostics

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      Pages 25-40

      Teaching Microfluidic Diagnostics Using Jell-O® Chips

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      Pages 41-54

      Fundamentals of Microfluidics for High School Students with No Prior Knowledge of Fluid Mechanics

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      Pages 55-63

      Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method

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      Pages 65-83

      Overview of the Microfluidic Diagnostics Commercial Landscape

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      Pages 85-102

      Practical Aspects of the Preparation and Filing of Patent Applications

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      Pages 103-112

      Introduction to In Vitro Diagnostic Device Regulatory Requirements

  3. Microfluidic Diagnostics: Fabrication and Manipulation Protocols

    1. Front Matter

      Pages 113-113

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      Pages 115-123

      Microfluidic Device Fabrication by Thermoplastic Hot-Embossing

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      Pages 125-140

      Introduction to Glass Microstructuring Techniques

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      Pages 141-151

      Glass Microstructure Capping and Bonding Techniques

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      Pages 153-168

      Rapid Prototyping of PDMS Devices Using SU-8 Lithography

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      Pages 169-184

      Microfluidic Interface Technology Based on Stereolithography for Glass-Based Lab-on-a-Chips

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      Pages 185-196

      Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays

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      Pages 197-205

      Thread Based Devices for Low-Cost Diagnostics

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      Pages 207-230

      Droplet-Based Microfluidics

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      Pages 231-240

      Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET

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      Pages 241-268

      Surface Treatments for Microfluidic Biocompatibility

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      Pages 269-281

      Superhydrophobicity for Antifouling Microfluidic Surfaces

  4. Microfluidic Diagnostics: Application Protocols

    1. Front Matter

      Pages 283-283

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      Pages 285-303

      The Application of Microfluidic Devices for Viral Diagnosis in Developing Countries

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      Pages 305-334

      Applications of Microfluidics for Molecular Diagnostics

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