Basic Cell Culture Protocols

Editors:

ISBN: 978-1-62703-127-1 (Print) 978-1-62703-128-8 (Online)

Table of contents (33 protocols)

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  1. Front Matter

    Pages i-xiv

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    Pages 1-13

    Detection of Mycoplasma Contaminations

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    Pages 15-26

    Eradication of Mycoplasma Contaminations

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    Pages 27-38

    STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination

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    Pages 39-60

    Classical and Molecular Cytogenetic Analysis

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    Pages 61-83

    Fluorescent In Situ Hybridization of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle

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    Pages 85-102

    The Development of T Lymphocytes in Fetal Thymus Organ Culture

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    Pages 103-113

    Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors

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    Pages 115-132

    In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells

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    Pages 133-149

    Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media

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    Pages 151-162

    Isolation and Characterization of Mouse Side Population Cells

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    Pages 163-179

    Stem Cell Identification by DyeCycle Violet Side Population Analysis

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    Pages 181-204

    Isolation and Characterization of Cancer Stem Cells In Vitro

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    Pages 205-224

    Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets

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    Pages 225-239

    Generation and Characterization of Murine Alternatively Activated Macrophages

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    Pages 241-256

    Human Long-Term Culture Initiating Cell Assay

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    Pages 257-266

    Long-Term Culture-Initiating Cell Assay for Mouse Cells

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    Pages 267-283

    Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells

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    Pages 285-300

    Studying Leukocyte Recruitment Under Flow Conditions

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    Pages 301-314

    Generation and Establishment of Murine Adherent Cell Lines

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    Pages 315-334

    Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture

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