Fungal Secondary Metabolism

Methods and Protocols

Editors:

ISBN: 978-1-62703-121-9 (Print) 978-1-62703-122-6 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-22

    Library Preparation and Data Analysis Packages for Rapid Genome Sequencing

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    Pages 23-45

    Bioinformatics Approaches and Software for Detection of Secondary Metabolic Gene Clusters

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    Pages 47-58

    Media and Growth Conditions for Induction of Secondary Metabolite Production

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    Pages 59-78

    Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays

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    Pages 79-96

    The “FERMEX” Method for Metabolite-Enriched Fungal Extracts

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    Pages 97-109

    The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites

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    Pages 111-119

    Detection and Purification of Non-ribosomal Peptide Synthetase Products in Neosartorya fischeri

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    Pages 121-131

    Production, Detection, and Purification of Clavine-Type Ergot Alkaloids

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    Pages 133-142

    Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid-Phase Microextraction-Gas Chromatography/Mass Spectrometry

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    Pages 143-161

    Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans

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    Pages 163-174

    Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis

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    Pages 175-182

    Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host

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    Pages 183-190

    Multi-Copy Genetic Screen in Aspergillus nidulans

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    Pages 191-205

    Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification

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    Pages 207-219

    Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra

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    Pages 221-236

    Chromatin Immunoprecipitation Analysis in Filamentous Fungi

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    Pages 237-249

    Targeted Proteomics for Metabolic Pathway Optimization

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    Pages 251-257

    Investigation of In Vivo Protein Interactions in Aspergillus Spores

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    Pages 259-266

    Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus

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    Pages 267-277

    Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi

  22. Back Matter

    Pages 287-288

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