Biolistic DNA Delivery

Methods and Protocols

Editors:

ISBN: 978-1-62703-109-7 (Print) 978-1-62703-110-3 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-16

      Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun

    3. No Access

      Book Protocol

      Pages 17-26

      Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery

    4. No Access

      Book Protocol

      Pages 27-44

      Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues

    5. No Access

      Book Protocol

      Pages 45-51

      Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-vacuum Gene Gun (HandyGun)

    6. No Access

      Book Protocol

      Pages 53-62

      HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies

    7. No Access

      Book Protocol

      Pages 63-74

      Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun

  3. Biolistic DNA Delivery in Nematodes

    1. Front Matter

      Pages 75-75

    2. No Access

      Book Protocol

      Pages 77-86

      Biolistic Transformation of Caenorhabditis elegans

    3. No Access

      Book Protocol

      Pages 87-102

      Improved Vectors for Selection of Transgenic Caenorhabditis elegans

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      Book Protocol

      Pages 103-115

      Biolistic Transformation of Brugia Malayi

  4. Biolistic DNA Delivery in Mammalian Cells In Vitro

    1. Front Matter

      Pages 117-117

    2. No Access

      Book Protocol

      Pages 119-132

      Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells

    3. No Access

      Book Protocol

      Pages 133-143

      Biolistic Transfection of Tumor Tissue Samples

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      Book Protocol

      Pages 145-155

      Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes

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      Book Protocol

      Pages 157-166

      Biolistic Transfection of Neurons in Organotypic Brain Slices

  5. Biolistic DNA Delivery in Mammalian Cells In Vivo

    1. Front Matter

      Pages 167-167

    2. No Access

      Book Protocol

      Pages 169-174

      Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun

    3. No Access

      Book Protocol

      Pages 175-188

      Chemokine Overexpression in the Skin by Biolistic DNA Delivery

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      Book Protocol

      Pages 189-198

      Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T-Cell Response by Administration of Chemotherapeutic Drugs

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      Book Protocol

      Pages 199-213

      Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter

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      Book Protocol

      Pages 215-220

      Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice

  6. Biolistic DNA Vaccination in Animal Disease Models

    1. Front Matter

      Pages 221-221

    2. No Access

      Book Protocol

      Pages 223-237

      Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets

    3. No Access

      Book Protocol

      Pages 239-267

      Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models

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