Phytoplasma

Methods and Protocols

Editors:

ISBN: 978-1-62703-088-5 (Print) 978-1-62703-089-2 (Online)

Table of contents (34 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-14

    The Phytoplasmas: An Introduction

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    Pages 15-32

    Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus

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    Pages 33-39

    Micropropagation and Maintenance of Phytoplasmas in Tissue Culture

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    Pages 41-46

    Dodder Transmission of Phytoplasmas

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    Pages 47-59

    Insect Maintenance and Transmission

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    Pages 61-72

    Capturing Insect Vectors of Phytoplasmas

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    Pages 73-85

    Insect Vector Transmission Assays

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    Pages 87-108

    Molecular Identification of Phytoplasma Vector Species

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    Pages 109-113

    Dienes’ Staining and Light Microscopy for Phytoplasma Visualization

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    Pages 115-121

    DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas

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    Pages 123-138

    Visualization of Phytoplasmas Using Electron Microscopy

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    Pages 139-145

    Automated DNA Extraction for Large Numbers of Plant Samples

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    Pages 147-158

    DNA Extraction from Arborescent Monocots and How to Deal with Other Challenging Hosts

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    Pages 159-171

    Nested PCR and RFLP Analysis Based on the 16S rRNA Gene

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    Pages 173-188

    PCR and RFLP Analyses Based on the Ribosomal Protein Operon

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    Pages 189-204

    Tuf and secY PCR Amplification and Genotyping of Phytoplasmas

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    Pages 205-215

    PCR Analysis of Phytoplasmas Based on the secA Gene

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    Pages 217-222

    Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains

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    Pages 223-232

    Microarrays for Universal Detection and Identification of Phytoplasmas

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    Pages 233-244

    T-RFLP for Detection and Identification of Phytoplasmas in Plants

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