Calcium Signaling Protocols

Editors:

ISBN: 978-1-62703-085-4 (Print) 978-1-62703-086-1 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xi

  2. General

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-36

      Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations

    3. No Access

      Book Protocol

      Pages 37-47

      Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2

  3. Specialist Measurement Systems

    1. Front Matter

      Pages 49-49

    2. No Access

      Book Protocol

      Pages 51-93

      Confocal Microscopy: Theory and Applications for Cellular Signaling

    3. No Access

      Book Protocol

      Pages 95-101

      Ratiometric Ca2+ Measurements Using the FlexStation®Scanning Fluorometer

    4. No Access

      Book Protocol

      Pages 103-109

      Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®)

    5. No Access

      Book Protocol

      Pages 111-120

      Ratiometric [Ca2+]i Measurements in Adherent Cell-Lines Using the NOVOstar Microplate Reader

  4. Measurement of Ca2+ Channel Activity

    1. Front Matter

      Pages 121-121

    2. No Access

      Book Protocol

      Pages 123-148

      Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones

    3. No Access

      Book Protocol

      Pages 149-160

      Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells

  5. Measurement of Ins(1,4,5)P3 and Ca2+ Release Form Intracellular Stores

    1. Front Matter

      Pages 161-161

    2. No Access

      Book Protocol

      Pages 163-174

      Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells

    3. No Access

      Book Protocol

      Pages 175-192

      Single-Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells

    4. No Access

      Book Protocol

      Pages 193-203

      Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay

  6. Specialist Measurement Techniques

    1. Front Matter

      Pages 205-205

    2. No Access

      Book Protocol

      Pages 207-216

      Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry

    3. No Access

      Book Protocol

      Pages 217-228

      Calcium Measurements from Whole Heart Using Rhod-2

    4. No Access

      Book Protocol

      Pages 229-238

      Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries

    5. No Access

      Book Protocol

      Pages 239-251

      Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration

    6. No Access

      Book Protocol

      Pages 253-271

      Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations

    7. No Access

      Book Protocol

      Pages 273-291

      Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes

    8. No Access

      Book Protocol

      Pages 293-306

      Chimeric G Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors

    9. No Access

      Book Protocol

      Pages 307-326

      Compartmentalizing Genetically Encoded Calcium Sensors

    10. No Access

      Book Protocol

      Pages 327-341

      Measurement of Cytosolic-Free Ca2+ in Plant Tissue

  7. Back Matter

    Pages 357-360

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