MicroRNA Protocols

Editors:

ISBN: 978-1-62703-082-3 (Print) 978-1-62703-083-0 (Online)

Table of contents (27 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-19

    The MicroRNA

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    Pages 21-34

    Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer

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    Pages 35-45

    A User-Friendly Computational Workflow for the Analysis of MicroRNA Deep Sequencing Data

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    Pages 47-67

    The RNA Gene Information: Retroelement-MicroRNA Entangling as the RNA Quantum Code

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    Pages 69-76

    Setting Up an Intronic miRNA Database

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    Pages 77-82

    Identify Intronic MicroRNA with Bioinformatics

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    Pages 83-90

    Experimental miRNA Target Validation

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    Pages 91-103

    Enrichment Analysis of miRNA Targets

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    Pages 105-116

    MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells

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    Pages 117-127

    Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay

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    Pages 129-141

    Expression Pattern Analysis of MicroRNAs in Caenorhabditis elegans

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    Pages 143-156

    Use of Viral Systems to Study miRNA-Mediated Regulation of Gene Expression in Human Cells

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    Pages 157-172

    Cloning, Expression, and Functional Analysis of Genomic miRNA Using Retroviral System in Cancer Cells

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    Pages 173-179

    Preparing Synaptoneurosomes from Adult Mouse Forebrain

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    Pages 181-194

    Isolation of Total RNA and Detection Procedures for miRNA Present in Bovine-Cultured Adipocytes and Adipose Tissues

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    Pages 195-207

    miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing

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    Pages 209-229

    Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs

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    Pages 231-245

    Vector-Free Methods for Manipulating miRNA Activity In Vitro and In Vivo

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    Pages 247-256

    MicroRNA Expression Profiling of Human-Induced Pluripotent and Embryonic Stem Cells

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    Pages 257-269

    MicroRNA Expression During Neuronal Differentiation of Human Teratocarcinoma NTera2D1 and Mouse Embryonic Carcinoma P19 Cells

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