Retinal Degeneration

Methods and Protocols

Editors:

ISBN: 978-1-62703-079-3 (Print) 978-1-62703-080-9 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Human Genetics

    1. Front Matter

      Pages 1-1

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      Pages 3-23

      Identification and Analysis of Inherited Retinal Disease Genes

  3. Mouse Models

    1. Front Matter

      Pages 25-25

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      Pages 27-39

      Mouse Models for Studies of Retinal Degeneration and Diseases

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      Pages 41-67

      Retinal Fundus Imaging in Mouse Models of Retinal Diseases

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      Pages 69-78

      Functional Phenotyping of Mouse Models with ERG

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      Pages 79-85

      Phenotyping of Mouse Models with OCT

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      Pages 87-97

      Light Damage as a Model of Retinal Degeneration

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      Pages 99-109

      N -Methyl-d-Aspartate (NMDA)-Mediated Excitotoxic Damage: A Mouse Model of Acute Retinal Ganglion Cell Damage

  4. Other Animal Models

    1. Front Matter

      Pages 111-111

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      Pages 113-125

      Generation of Transgenic X. laevis Models of Retinal Degeneration

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      Pages 127-137

      Analysis of Photoreceptor Degeneration in the Zebrafish Danio rerio

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      Pages 139-160

      Analysis of Optokinetic Response in Zebrafish by Computer-Based Eye Tracking

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      Pages 161-182

      Analysis of the Drosophila Compound Eye with Light and Electron Microscopy

  5. In Situ Analyses

    1. Front Matter

      Pages 183-183

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      Pages 185-199

      Cell-Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy

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      Pages 201-205

      A Method of Horizontally Sliced Preparation of the Retina

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      Pages 207-213

      Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL

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      Pages 215-226

      High-Throughput RNA In Situ Hybridization in Mouse Retina

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      Pages 227-243

      Assessment of Mitochondrial Damage in Retinal Cells and Tissues Using Quantitative Polymerase Chain Reaction for Mitochondrial DNA Damage and Extracellular Flux Assay for Mitochondrial Respiration Activity

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      Pages 245-254

      Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells In Situ

  6. Tissue Culture and Cell Models

    1. Front Matter

      Pages 255-255

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      Pages 257-270

      Ca2+ Microfluorimetry in Retinal Müller Glial Cells

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      Pages 271-283

      Functional Analysis of Retinal Microglia and Their Effects on Progenitors

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      Pages 285-295

      Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture

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