Methods and Protocols


ISBN: 978-1-62703-037-3 (Print) 978-1-62703-038-0 (Online)
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Table of contents (45 protocols)

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  1. Front Matter

    Pages i-xvii

  2. Semen Analysis Methods

    1. Front Matter

      Pages 1-1

    2. Book Protocol

      Pages 3-12

      Methods for Sperm Concentration Determination

    3. Book Protocol

      Pages 13-19

      Methods of Sperm Vitality Assessment

    4. Book Protocol

      Pages 21-25

      The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity

    5. Book Protocol

      Pages 27-37

      Sperm Morphology Classification: A Rational Method for Schemes Adopted by the World Health Organization

    6. Book Protocol

      Pages 39-50

      Sperm Morphology Assessment Using Strict (Tygerberg) Criteria

    7. Book Protocol

      Pages 51-60

      Methods for Direct and Indirect Antisperm Antibody Testing

    8. Book Protocol

      Pages 61-75

      Manual Methods for Sperm Motility Assessment

    9. Book Protocol

      Pages 77-87

      Computer-Aided Sperm Analysis (CASA) of Sperm Motility and Hyperactivation

  3. Tests of Sperm Function

    1. Front Matter

      Pages 89-89

    2. Book Protocol

      Pages 91-102

      The Hemizona Assay for Assessment of Sperm Function

    3. Book Protocol

      Pages 103-111

      The Sperm Penetration Assay for the Assessment of Fertilization Capacity

    4. Book Protocol

      Pages 113-118

      Methods for the Assessment of Sperm Capacitation and Acrosome Reaction Excluding the Sperm Penetration Assay

  4. Sperm DNA Damage Testing

    1. Front Matter

      Pages 119-119

    2. Book Protocol

      Pages 121-136

      Sperm DNA Fragmentation Analysis Using the TUNEL Assay

    3. Book Protocol

      Pages 137-146

      Sperm DNA Damage Measured by Comet Assay

    4. Book Protocol

      Pages 147-164

      Sperm Chromatin Structure Assay (SCSA®)

  5. Advanced Clinical Testing

    1. Front Matter

      Pages 165-165

    2. Book Protocol

      Pages 167-173

      Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization

    3. Book Protocol

      Pages 175-186

      Flow Cytometric Methods for Sperm Assessment

    4. Book Protocol

      Pages 187-204

      Human Y Chromosome Microdeletion Analysis by PCR Multiplex Protocols Identifying only Clinically Relevant AZF Microdeletions

  6. Sperm Preparation and Selection Techniques

    1. Front Matter

      Pages 207-207

    2. Book Protocol

      Pages 209-215

      Sperm Cryopreservation Methods

    3. Book Protocol

      Pages 217-226

      Density Gradient Separation of Sperm for Artificial Insemination

    4. Book Protocol

      Pages 227-240

      Recovery, Isolation, Identification, and Preparation of Spermatozoa from Human Testis

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