Genomic Imprinting

Methods and Protocols

Editors:

ISBN: 978-1-62703-010-6 (Print) 978-1-62703-011-3 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. Parent-of-Origin Effects

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-19

      Uniparental Embryos in the Study of Genomic Imprinting

    3. No Access

      Book Protocol

      Pages 21-48

      Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species

    4. No Access

      Book Protocol

      Pages 49-59

      Generation of Trophoblast Stem Cells

    5. No Access

      Book Protocol

      Pages 61-66

      Immunomagnetic Purification of Murine Primordial Germ Cells

  3. Identifying Imprinted Genes

    1. Front Matter

      Pages 67-67

    2. No Access

      Book Protocol

      Pages 69-78

      Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA

    3. No Access

      Book Protocol

      Pages 79-88

      Identification of Imprinted Loci by Transcriptome Sequencing

    4. No Access

      Book Protocol

      Pages 89-134

      Data Mining as a Discovery Tool for Imprinted Genes

  4. Identifying the Regulatory Features of Imprinted Domains

    1. Front Matter

      Pages 135-135

    2. No Access

      Book Protocol

      Pages 137-146

      Engineering of Large Deletions and Duplications In Vivo

  5. Epigenetics of Imprinted Regions

    1. Front Matter

      Pages 147-147

    2. No Access

      Book Protocol

      Pages 149-158

      Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells

    3. No Access

      Book Protocol

      Pages 159-172

      Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains

    4. No Access

      Book Protocol

      Pages 173-185

      Quantitative Chromosome Conformation Capture

    5. No Access

      Book Protocol

      Pages 187-197

      Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing

  6. Analysis of Imprinted Expression

    1. Front Matter

      Pages 199-199

    2. No Access

      Book Protocol

      Pages 201-209

      Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies

    3. No Access

      Book Protocol

      Pages 211-218

      Generation of cDNA Libraries from RNP-Derived Regulatory Noncoding RNAs

    4. No Access

      Book Protocol

      Pages 219-228

      Co-Immunoprecipitation of Long Noncoding RNAs

  7. Imprinting in Plants

    1. Front Matter

      Pages 229-229

    2. No Access

      Book Protocol

      Pages 231-247

      Specialized Technologies for Epigenetics in Plants

  8. Evolution of Imprinted Genes

    1. Front Matter

      Pages 249-249

    2. No Access

      Book Protocol

      Pages 251-262

      Computational Studies of Imprinted Genes

    3. No Access

      Book Protocol

      Pages 263-275

      Insights on Imprinting from Beyond Mice and Men

    4. No Access

      Book Protocol

      Pages 277-294

      Nonmammalian Parent-of-Origin Effects

  9. Back Matter

    Pages 295-297