Helicobacter Species

Methods and Protocols

Editors:

ISBN: 978-1-62703-004-5 (Print) 978-1-62703-005-2 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-x

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    Pages 1-5

    Introduction

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    Pages 7-10

    Helicobacter pylori: An Overview

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    Pages 11-15

    Perspectives on Methodology for In Vitro Culture of Helicobacter pylori

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    Pages 17-27

    Successful Culture Techniques for Helicobacter Species: General Culture Techniques for Helicobacter pylori

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    Pages 29-35

    Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures from Infected Rodents

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    Pages 37-40

    Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis

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    Pages 41-50

    The Helicobacter pylori cag Pathogenicity Island

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    Pages 51-59

    Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori

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    Pages 61-68

    A Method for Short-Term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori

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    Pages 69-76

    Cell Culture-Based Assays to Test for Bacterial Adherence and Internalization

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    Pages 77-88

    Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence

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    Pages 89-98

    Rodent Models of Helicobacter Infection, Inflammation, and Disease

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    Pages 99-107

    Bacterial Culture and Inoculation of Mice (Simple Infection)

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    Pages 109-112

    Adoptive Transfer of Splenocytes to Immunocompromised Mice

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    Pages 113-116

    Isolation of Gastric Lamina Propria Leukocytes

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    Pages 117-118

    Delayed-Type Hypersensitivity Determination

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    Pages 119-129

    Necropsy, Blood, Tissue Collection, and mRNA Isolation for Detection of Host Cytokine Gene Expression

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    Pages 131-142

    Animal Models of Helicobacter-Induced Disease: Methods to Successfully Infect the Mouse

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    Pages 143-156

    Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice

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    Pages 157-173

    Mouse Models of Helicobacter-Induced Gastric Cancer: Use of Cocarcinogens

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