DNA Repair Protocols

Editors:

ISBN: 978-1-61779-997-6 (Print) 978-1-61779-998-3 (Online)

Table of contents (40 protocols)

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  1. Front Matter

    Pages i-xvii

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    Pages 1-6

    DNA Repair: Front and Center and Not Going Away!

  3. Identification of Repair Genes and Generation of Repair Mutants

    1. Front Matter

      Pages 7-7

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      Book Protocol

      Pages 9-26

      Identification of Genes Required for Damage Survival Using a Cell-Based RNAi Screen Against the Drosophila Genome

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      Pages 27-38

      Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans Using Genome-Wide RNAi Screening

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      Pages 39-49

      Establishment of the DNA Repair-Defective Mutants in DT40 Cells

  4. Assessing DNA Damage and Repair

    1. Front Matter

      Pages 51-51

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      Pages 53-78

      Electrophoretic Mobility Shift Assays for Protein–DNA Complexes Involved in DNA Repair

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      Pages 79-90

      The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair

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      Pages 91-100

      Detection of DNA Damage by Comet Fluorescence In Situ Hybridization

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      Pages 101-109

      Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assay

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      Pages 111-132

      Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells Using Quantitative PCR

  5. Repair of Incorrect or Damaged DNA Bases

    1. Front Matter

      Pages 133-133

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      Pages 135-147

      In Vitro DNA Mismatch Repair in Human Cells

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      Pages 149-162

      Eukaryotic DNA Mismatch Repair In Vitro

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      Pages 163-175

      Immuno-Slot Blot Assay for Detection of UVR-Mediated DNA Damage

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      Pages 177-187

      Quantification of DNA Photoproducts in Mammalian Cell DNA Using Radioimmunoassay

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      Pages 189-202

      Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-Mediated PCR

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      Pages 203-219

      Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies

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      Pages 221-243

      Replication-Coupled DNA Interstrand Cross-Link Repair in Xenopus Egg Extracts

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      Pages 245-262

      In Vitro Base Excision Repair Using Mammalian Cell Extracts

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      Pages 263-277

      Using Arabidopsis Cell Extracts to Monitor Repair of DNA Base Damage In Vitro

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      Pages 279-287

      In Vitro Monitoring of Base Excision Repair in Saccharomyces cerevisiae

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      Pages 289-304

      Mitochondrial Base Excision Repair Assays

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