Plant Salt Tolerance

Methods and Protocols

Editors:

ISBN: 978-1-61779-985-3 (Print) 978-1-61779-986-0 (Online)

Table of contents (28 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Microelectrode Techniques

    1. Front Matter

      Pages 1-1

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      Pages 3-18

      Patch-Clamp Protocols to Study Cell Ionic Homeostasis Under Saline Conditions

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      Pages 19-33

      Studying Plant Salt Tolerance with the Voltage Clamp Technique

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      Pages 35-66

      Using the Multifunctional Xylem Probe for in situ Studies of Plant Water and Ion Relations Under Saline Conditions

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      Pages 67-77

      Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes

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      Pages 79-100

      Single-Cell Sampling and Analysis (SiCSA)

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      Pages 101-117

      Non-invasive Flux Measurements Using Microsensors: Theory, Limitations, and Systems

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      Pages 119-134

      Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-invasive Microelectrode Measuring MIFE Technique

  3. Imaging Techniques

    1. Front Matter

      Pages 135-135

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      Pages 137-148

      Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX): An Important Technique Useful for Cell-Specific Localization of Salt

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      Pages 149-161

      Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research

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      Pages 163-171

      Cytosolic Ca2+ Determinations in Studying Plant Responses to Salinity and Oxidative Stress

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      Pages 173-189

      Infrared Thermography in Plant Phenotyping for Salinity Tolerance

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      Pages 191-200

      In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy

  4. Biochemical Assays

    1. Front Matter

      Pages 201-201

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      Pages 203-215

      Metabolomics for Salinity Research

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      Pages 217-223

      Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping

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      Pages 225-236

      Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues

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      Pages 237-250

      Quantification of the Antioxidant Activity in Salt-Stressed Tissues

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      Pages 251-261

      Quantification of Abscisic Acid, Cytokinin, and Auxin Content in Salt-Stressed Plant Tissues

  5. Molecular Techniques

    1. Front Matter

      Pages 263-263

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      Pages 265-276

      Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Specific Responses to Salinity Stress in Arabidopsis and Rice

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      Pages 277-290

      Transformation Using Controlled cDNA Overexpression System

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      Pages 291-303

      Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress

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