Progenitor Cells

Methods and Protocols

Editors:

ISBN: 978-1-61779-979-2 (Print) 978-1-61779-980-8 (Online)

Table of contents (28 protocols)

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  1. Front Matter

    Pages i-xiv

  2. In vitro Culture Systems

    1. Front Matter

      Pages 1-1

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      Pages 3-14

      Isolation of Satellite Cells from Single Muscle Fibers from Young, Aged, or Dystrophic Muscles

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      Pages 15-22

      Terminal Differentiation of Human Epidermal Stem Cells on Micro-patterned Substrates

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      Pages 23-30

      Isolation, Culture, and Potentiality Assessment of Lung Alveolar Stem Cells

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      Pages 31-45

      Three-Dimensional In Vitro Culture Techniques for Mesenchymal Stem Cells

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      Pages 47-57

      Isolation of Adult Stem Cells and Their Differentiation to Schwann Cells

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      Pages 59-79

      Functional Purification of Human and Mouse Mammary Stem Cells

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      Pages 81-96

      Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells

  3. Non-mammalian Model Systems

    1. Front Matter

      Pages 97-97

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      Pages 99-110

      Transcriptome Analysis of Drosophila Neural Stem Cells

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      Pages 111-125

      Live Imaging for Studying Asymmetric Cell Division in the C. elegans Embryo

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      Pages 127-139

      Tol2-Mediated Gene Transfer and In Ovo Electroporation of the Otic Placode: A Powerful and Versatile Approach for Investigating Embryonic Development and Regeneration of the Chicken Inner Ear

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      Pages 141-155

      Labeling Primitive Myeloid Progenitor Cells in Xenopus

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      Pages 157-165

      Identification of Oocyte Progenitor Cells in the Zebrafish Ovary

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      Pages 167-179

      FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms

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      Pages 181-195

      Clonal and Lineage Analysis of Melanocyte Stem Cells and Their Progeny in the Zebrafish

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      Pages 197-202

      Reconstitution of the Central Nervous System During Salamander Tail Regeneration from the Implanted Neurospheres

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      Pages 203-215

      Following the Fate of Neural Progenitors by Homotopic/Homochronic Grafts in Xenopus Embryos

  4. Mammalian Model Systems

    1. Front Matter

      Pages 217-217

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      Pages 219-229

      Analyzing the Angiogenic Potential of Gr-1+CD11b+ Immature Myeloid Cells from Murine Wounds

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      Pages 231-242

      In Vivo Imaging of Hematopoietic Stem Cells in the Bone Marrow Niche

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      Pages 243-261

      Characterizing the Phenotype of Murine Epidermal Progenitor Cells: Complementary Whole-Mount Visualization and Flow Cytometry Strategies

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      Pages 263-274

      Murine Aggregation Chimeras and Wholemount Imaging in Airway Stem Cell Biology

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