Antibody Engineering

Methods and Protocols, Second Edition

Editors:

ISBN: 978-1-61779-973-0 (Print) 978-1-61779-974-7 (Online)

Table of contents (40 protocols)

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  1. Front Matter

    Pages i-xvi

  2. In Silico Methods

    1. Front Matter

      Pages 1-1

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      Pages 3-37

      Use of IMGT® Databases and Tools for Antibody Engineering and Humanization

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      Pages 39-55

      Computer-Assisted Modeling of Antibody Variable Domains

  3. Generation of Diversity

    1. Front Matter

      Pages 57-57

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      Pages 59-71

      Cloning Single-Chain Antibody Fragments (ScFv) from Hyrbidoma Cells

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      Pages 73-83

      Human In-Cell scFv Library from Infiltrating B Cell

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      Pages 85-107

      Construction of Human Naive Antibody Gene Libraries

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      Pages 109-122

      Synthetic Customized scFv Libraries

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      Pages 123-144

      Selection of Stable scFv Antibodies by Phage Display

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      Pages 145-176

      Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs

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      Pages 177-194

      Generation and Isolation of Target-Specific Single-Domain Antibodies from Shark Immune Repertoires

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      Pages 195-209

      Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis

  4. Selections of Lead Candidate

    1. Front Matter

      Pages 211-211

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      Pages 213-224

      Phage Display and Selections on Purified Antigens

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      Pages 225-235

      Phage Display and Selections on Cells

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      Pages 237-245

      Humanization by CDR Grafting and Specificity-Determining Residue Grafting

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      Pages 247-257

      Humanization by Guided Selections

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      Pages 259-280

      Selection of Antibody Fragments by Yeast Display

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      Pages 281-292

      Evolution of Antibodies In Vitro by Ribosome Display

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      Pages 293-302

      Mammalian Cell Surface Display of Full Length IgG

  5. Production of Recombinant Antibodies

    1. Front Matter

      Pages 303-303

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      Pages 305-324

      Production of Antibody Fragments in Escherichia coli

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      Pages 325-340

      Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastoris

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      Pages 341-358

      Monoclonal Antibody Expression in Mammalian Cells

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