Embryo Culture

Methods and Protocols

Editors:

ISBN: 978-1-61779-970-9 (Print) 978-1-61779-971-6 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-x

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    Pages 1-18

    Historical Background of Gamete and Embryo Culture

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    Pages 19-26

    Utility of Animal Models for Human Embryo Culture Development: Rodents

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    Pages 27-37

    Utility of Animal Models for Human Embryo Culture Development: Domestic Species

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    Pages 39-59

    Utility of Animal Models for Human Embryo Culture: Nonhuman Primates

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    Pages 61-80

    Media Composition: Salts and Osmolality

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    Pages 81-96

    Media Composition: Energy Sources and Metabolism

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    Pages 97-106

    Media Composition: Amino Acids and Cellular Homeostasis

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    Pages 107-127

    Media Composition: Macromolecules and Embryo Growth

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    Pages 129-159

    Media Composition: Antioxidants/Chelators and Cellular Function

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    Pages 161-175

    Media Composition: pH and Buffers

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    Pages 177-198

    Media Composition: Growth Factors

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    Pages 199-209

    Culture Systems: Single Step

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    Pages 211-230

    Culture Systems: Sequential

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    Pages 231-247

    Culture Systems: Embryo Co-Culture

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    Pages 249-272

    Culture Systems: Low-Oxygen Culture

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    Pages 273-312

    Culture Systems: Embryo Density

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    Pages 313-324

    Culture Systems: Air Quality

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    Pages 325-331

    Culture Systems: Mineral Oil Overlay

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    Pages 333-354

    Culture Systems: Physiological and Environmental Factors That Can Affect the Outcome of Human ART

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    Pages 355-365

    Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics)

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