Single Domain Antibodies

Methods and Protocols

Editors:

ISBN: 978-1-61779-967-9 (Print) 978-1-61779-968-6 (Online)

Table of contents (36 protocols)

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  1. Front Matter

    Pages i-xv

  2. Overview of Single Domain Antibodies

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-13

      From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small

    3. No Access

      Book Protocol

      Pages 15-26

      Introduction to Heavy Chain Antibodies and Derived Nanobodies

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      Book Protocol

      Pages 27-36

      Overview and Discovery of IgNARs and Generation of VNARs

  3. Single Domain Antibody Library Construction

    1. Front Matter

      Pages 37-37

    2. No Access

      Book Protocol

      Pages 39-63

      Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies

    3. No Access

      Book Protocol

      Pages 65-78

      Preparation of a Naïve Library of Camelid Single Domain Antibodies

  4. Selection of Single Domain Antibodies

    1. Front Matter

      Pages 79-79

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      Book Protocol

      Pages 81-104

      Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation

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      Book Protocol

      Pages 105-124

      Semiautomated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens

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      Book Protocol

      Pages 125-134

      Pichia Surface Display: A Tool for Screening Single Domain Antibodies

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      Book Protocol

      Pages 135-150

      Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method

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      Book Protocol

      Pages 151-173

      Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies

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      Book Protocol

      Pages 175-182

      Selection of Functional Single Domain Antibody Fragments for Interfering with Protein–Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System

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      Book Protocol

      Pages 183-198

      Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization

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      Book Protocol

      Pages 199-209

      Selection of VHHs Under Application Conditions

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      Book Protocol

      Pages 211-239

      Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies

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      Book Protocol

      Pages 241-253

      Selection of VHH Antibody Fragments That Recognize Different Aβ Depositions Using Complex Immune Libraries

  5. Expression of Single Domain Antibodies and Derivatives

    1. Front Matter

      Pages 225-225

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      Book Protocol

      Pages 257-275

      Expression of Single-Domain Antibodies in Bacterial Systems

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      Book Protocol

      Pages 277-286

      Expression of VHHs in Saccharomyces cerevisiae

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      Book Protocol

      Pages 287-303

      Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells

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      Book Protocol

      Pages 305-324

      Production of Camel-Like Antibodies in Plants

  6. Improvement and Applications of Single Domain Antibodies

    1. Front Matter

      Pages 325-325

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      Book Protocol

      Pages 327-353

      Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains

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