Liver Proteomics

Methods and Protocols

Editors:

ISBN: 978-1-61779-958-7 (Print) 978-1-61779-959-4 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-16

    Cell Surface Capturing Technologies for the Surfaceome Discovery of Hepatocytes

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    Pages 17-28

    An On-Target Desalting and Concentration Sample Preparation Protocol for MALDI-MS and MS/MS Analysis

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    Pages 29-41

    Plasma Membrane Isolation Using Immobilized Concanavalin A Magnetic Beads

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    Pages 43-62

    Analysis of the Cattle Liver Proteome by High-Sensitive Liquid Chromatography Coupled with Mass Spectrometry Method

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    Pages 63-82

    Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450

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    Pages 83-96

    Assessing Heterogeneity of Peroxisomes: Isolation of Two Subpopulations from Rat Liver

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    Pages 97-111

    Purification and Proteomic Analysis of Liver Membrane Skeletons

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    Pages 113-123

    Liver Plasma Membranes: An Effective Method to Analyze Membrane Proteome

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    Pages 125-140

    Quantitative Analysis of Liver Golgi Proteome in the Cell Cycle

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    Pages 141-150

    Glycoproteins and Glycosylation: Apolipoprotein C3 Glycoforms by Top-Down MALDI-TOF Mass Spectrometry

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    Pages 151-163

    Phosphoproteomic Analysis of Liver Homogenates

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    Pages 165-180

    A Combination of Affinity Chromatography, 2D DIGE, and Mass Spectrometry to Analyze the Phosphoproteome of Liver Progenitor Cells

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    Pages 181-204

    Identifying Acetylated Proteins in Mitosis

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    Pages 205-225

    Comparative Proteome Analysis of a Human Liver Cell Line Stably Transfected with Hepatitis D Virus Full-Length cDNA

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    Pages 227-239

    Identification of New Autoimmune Hepatitis-Specific Autoantigens by Using Protein Microarray Technology

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    Pages 241-258

    Proteomics Analysis of Human Nonalcoholic Fatty Liver

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    Pages 259-277

    Proteomic Methods for Biomarker Discovery in a Rat Model of Alcohol Steatosis

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    Pages 279-294

    Targeted Mass Spectrometry-Based Metabolomic Profiling Through Multiple Reaction Monitoring of Liver and Other Biological Matrices

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    Pages 295-310

    Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma

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    Pages 311-319

    Combinatorial Peptide Ligand Libraries to Discover Liver Disease Biomarkers in Plasma Samples

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