Biomass Conversion

Methods and Protocols

Editors:

ISBN: 978-1-61779-955-6 (Print) 978-1-61779-956-3 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xiv

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    Book Protocol

    Pages 1-8

    Challenges for Assessing the Performance of Biomass Degrading Biocatalysts

  3. Biomass Feedstock and Cellulose

    1. Front Matter

      Pages 9-9

    2. No Access

      Book Protocol

      Pages 11-22

      Overview of Computer Modeling of Cellulose

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      Book Protocol

      Pages 23-30

      Imaging Cellulose Using Atomic Force Microscopy

    4. No Access

      Book Protocol

      Pages 31-47

      Preservation and Preparation of Lignocellulosic Biomass Samples for Multi-scale Microscopy Analysis

    5. No Access

      Book Protocol

      Pages 49-60

      Coherent Raman Microscopy Analysis of Plant Cell Walls

    6. No Access

      Book Protocol

      Pages 61-72

      Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling

    7. No Access

      Book Protocol

      Pages 73-82

      Immunological Approaches to Plant Cell Wall and Biomass Characterization: Immunolocalization of Glycan Epitopes

    8. No Access

      Book Protocol

      Pages 83-89

      A Method to Evaluate Biomass Accessibility in Wet State Based on Thermoporometry

  4. Plant Cell Wall

    1. Front Matter

      Pages 91-91

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      Book Protocol

      Pages 93-99

      Cellulase Processivity

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      Pages 101-107

      A Simple Method for Determining Specificity of Carbohydrate-Binding Modules for Purified and Crude Insoluble Polysaccharide Substrates

    4. No Access

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      Pages 109-118

      Bacterial Cadherin Domains as Carbohydrate Binding Modules: Determination of Affinity Constants to Insoluble Complex Polysaccharides

    5. No Access

      Book Protocol

      Pages 119-127

      Affinity Electrophoresis as a Method for Determining Substrate-Binding Specificity of Carbohydrate-Active Enzymes for Soluble Polysaccharides

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      Book Protocol

      Pages 129-140

      Single-Molecule Tracking of Carbohydrate-Binding Modules on Cellulose Using Fluorescence Microscopy

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      Book Protocol

      Pages 141-151

      Bioprospecting Metagenomics for New Glycoside Hydrolases

    8. No Access

      Book Protocol

      Pages 153-168

      Anaerobic High-Throughput Cultivation Method for Isolation of Thermophiles Using Biomass-Derived Substrates

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      Book Protocol

      Pages 169-180

      Assessing the Protein Concentration in Commercial Enzyme Preparations

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      Book Protocol

      Pages 181-195

      Reducing the Effect of Variable Starch Levels in Biomass Recalcitrance Screening

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      Book Protocol

      Pages 197-211

      Analysis of Transgenic Glycoside Hydrolases Expressed in Plants: T. reesei CBH I and A. cellulolyticus EI

  5. Lignins and Hemicelluloses

    1. Front Matter

      Pages 213-213

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      Book Protocol

      Pages 215-228

      Structural Characterization of the Heteroxylans from Poplar and Switchgrass

    3. No Access

      Book Protocol

      Pages 229-250

      Laser Microdissection and Genetic Manipulation Technologies to Probe Lignin Heterogeneity and Configuration in Plant Cell Walls

  6. Back Matter

    Pages 269-275

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