Microbial Carotenoids From Fungi

Methods and Protocols

Editors:

ISBN: 978-1-61779-917-4 (Print) 978-1-61779-918-1 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-40

    Essential Role of Genetics in the Advancement of Biotechnology

  3. No Access

    Book Protocol

    Pages 41-59

    Microbial Carotenoids

  4. No Access

    Book Protocol

    Pages 61-74

    Biosynthesis, Extraction, Purification, and Analysis of Trisporoid Sexual Communication Compounds from Mated Cultures of Blakeslea trispora

  5. No Access

    Book Protocol

    Pages 75-84

    Isolation of Mutants and Construction of Intersexual Heterokaryons of Blakeslea trispora

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    Book Protocol

    Pages 85-107

    Molecular Tools for Carotenogenesis Analysis in the Zygomycete Mucor circinelloides

  7. No Access

    Book Protocol

    Pages 109-122

    Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides

  8. No Access

    Book Protocol

    Pages 123-132

    Integration of a Bacterial β-Carotene Ketolase Gene into the Mucor circinelloides Genome by the Agrobacterium tumefaciens-Mediated Transformation Method

  9. No Access

    Book Protocol

    Pages 133-151

    Metabolic Engineering of Mucor circinelloides for Zeaxanthin Production

  10. No Access

    Book Protocol

    Pages 153-159

    Bioengineering of Oleaginous Yeast Yarrowia lipolytica for Lycopene Production

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    Book Protocol

    Pages 161-169

    Peroxisome Targeting of Lycopene Pathway Enzymes in Pichia pastoris

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    Book Protocol

    Pages 171-182

    Production, Extraction, and Quantification of Astaxanthin by Xanthophyllomyces dendrorhous or Haematococcus pluvialis: Standardized Techniques

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    Book Protocol

    Pages 183-194

    Isolation and Selection of New Astaxanthin Producing Strains of Xanthophyllomyces dendrorhous

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    Book Protocol

    Pages 195-205

    Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements in Xanthophyllomyces dendrorhous

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    Book Protocol

    Pages 207-217

    Isolation of Carotenoid Hyperproducing Mutants of Xanthophyllomyces dendrorhous (Phaffia rhodozyma) by Flow Cytometry and Cell Sorting

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    Book Protocol

    Pages 219-234

    Generation of Astaxanthin Mutants in Xanthophyllomyces dendrorhous Using a Double Recombination Method Based on Hygromycin Resistance

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    Book Protocol

    Pages 235-249

    Genetic Manipulation of Xanthophyllomyces dendrorhous and Phaffia rhodozyma

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    Book Protocol

    Pages 251-262

    DNA Assembler Method for Construction of Zeaxanthin-Producing Strains of Saccharomyces cerevisiae

  19. No Access

    Book Protocol

    Pages 263-274

    Neurosporaxanthin Production by Neurospora and Fusarium

  20. No Access

    Book Protocol

    Pages 275-283

    Production of Torularhodin, Torulene, and β-Carotene by Rhodotorula Yeasts

  21. Back Matter

    Pages 285-290