Quantitative Methods in Proteomics

Editors:

ISBN: 978-1-61779-884-9 (Print) 978-1-61779-885-6 (Online)

Table of contents (32 protocols)

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  1. Front Matter

    Pages i-xv

  2. Planning a Proteomic Experiment

    1. Front Matter

      Pages 1-1

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      Book Protocol

      Pages 3-21

      Important Issues in Planning a Proteomics Experiment: Statistical Considerations of Quantitative Proteomic Data

  3. Quantitative Proteomics: Gel-Based Methods

    1. Front Matter

      Pages 23-23

    2. No Access

      Book Protocol

      Pages 25-35

      The Whereabouts of 2D Gels in Quantitative Proteomics

    3. No Access

      Book Protocol

      Pages 37-46

      Proteome Analysis with Classical 2D-PAGE

    4. No Access

      Book Protocol

      Pages 47-59

      Fast and Sensitive Coomassie Staining in Quantitative Proteomics

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      Book Protocol

      Pages 61-73

      Silver Staining of 2D Electrophoresis Gels

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      Book Protocol

      Pages 75-82

      Differential Proteome Analysis Using 2D-DIGE

  4. Quantitative Proteomics: MS-Based Methods

    1. Front Matter

      Pages 83-83

    2. No Access

      Book Protocol

      Pages 85-100

      Quantitative Mass Spectrometry-Based Proteomics: An Overview

    3. No Access

      Book Protocol

      Pages 101-113

      Robust Workflow for iTRAQ-Based Peptide and Protein Quantification

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      Book Protocol

      Pages 115-127

      Relative Protein Quantification by MS/MS Using the Tandem Mass Tag Technology

    5. No Access

      Book Protocol

      Pages 129-141

      A Rapid Approach for Isobaric Peptide Termini Labeling

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      Book Protocol

      Pages 143-153

      Isotope-Coded Protein Label

    7. No Access

      Book Protocol

      Pages 155-173

      Hydroponic Isotope Labeling of Entire Plants and High-Performance Mass Spectrometry for Quantitative Plant Proteomics

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      Book Protocol

      Pages 175-199

      In Vivo Quantitative Proteome Profiling: Planning and Evaluation of SILAC Experiments

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      Book Protocol

      Pages 201-221

      SILAC for the Study of Mammalian Cell Lines and Yeast Protein Complexes

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      Book Protocol

      Pages 223-240

      Post-digestion 18O Exchange/Labeling for Quantitative Shotgun Proteomics of Membrane Proteins

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      Book Protocol

      Pages 241-248

      Application of Label-Free Proteomics for Differential Analysis of Lung Carcinoma Cell Line A549

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      Book Protocol

      Pages 249-265

      Absolute Quantification of Proteins Using Standard Peptides and Multiple Reaction Monitoring

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      Book Protocol

      Pages 267-293

      Absolute Multiplexed Protein Quantification Using QconCAT Technology

    14. No Access

      Book Protocol

      Pages 295-319

      A Practical Guide to the FLEXIQuant Method

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      Book Protocol

      Pages 321-341

      Label-Free Protein Quantitation Using Weighted Spectral Counting

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