Antiviral Resistance in Plants

Methods and Protocols

Editors:

ISBN: 978-1-61779-881-8 (Print) 978-1-61779-882-5 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-16

    A Historical Overview of RNAi in Plants

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    Pages 17-38

    RNA Silencing and Antiviral Defense in Plants

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    Pages 39-56

    Biological Chemistry of Virus-Encoded Suppressors of RNA Silencing: An Overview

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    Pages 57-67

    Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules

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    Pages 69-81

    Strategies for Viral Cross Protection in Plants

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    Pages 83-92

    Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing

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    Pages 93-112

    Virus-Induced Gene Silencing in Hexaploid Wheat Using Barley Stripe Mosaic Virus Vectors

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    Pages 113-122

    Characterization of Plant Virus-Encoded Gene Silencing Suppressors

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    Pages 123-137

    Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing

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    Pages 139-154

    Modeling Antiviral Resistance in Plants

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    Pages 155-172

    Isolation and Detection of Small RNAs from Plant Tissues

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    Pages 173-189

    Isolation and Analysis of Small RNAs from Virus-Infected Plants

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    Pages 191-208

    Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance

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    Pages 209-222

    Artificial MicroRNAs for Plant Virus Resistance

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    Pages 223-239

    Isolation and Biochemical Analysis of Plant Small RNAs

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    Pages 241-252

    Isolation and Analysis of Plant RNA-Dependent RNA Polymerases

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    Pages 253-271

    Plant Viroids: Isolation, Characterization/Detection, and Analysis

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    Pages 273-286

    Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing

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    Pages 287-297

    Virus-Induced Gene Silencing in Soybean

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    Pages 299-308

    Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in “Dark Green” Islands

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